Browsing by Author "Santos-Silva, Alice"
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- Adipokine gene single-nucleotide polymorphisms in portuguese obese adolescents: associations with plasma concentrations of adiponectin, resistin, IL-6, IL-1β, and TNF-αPublication . Nascimento, Henrique; Vieira, Emília; Coimbra, Susana; Catarino, Cristina; Costa, Elísio; Bronze-da-Rocha, Elsa; Rocha-Pereira, Petronila; Carvalho, Márcia; Mansilha, Helena Ferreira; Rêgo, Carla; Santos, Rosário dos; Santos-Silva, Alice; Belo, LuísBackground: The genetic contribution to obesity and to circulating adipokine levels has not been completely clarified. We aimed to evaluate adipokine genes' single-nucleotide polymorphism (SNP) prevalence and its association with circulating adipokine levels and risk factors for cardiovascular disease in an obese Portuguese pediatric population. Methods: Two hundred forty-eight obese adolescents (mean age 13.4 years old; 47.2% females) participated in a cohort study. We screened 12 SNPs by direct sequencing in five adipokine genes: adiponectin (ADIPOQ: rs16861194, rs17300539, rs266729, rs2241766, rs1501299), interleukin-1β (IL-1β; rs1143627), IL-6 (IL-6; rs1800795), tumor necrosis factor-α (TNF-α; rs1800629), and resistin (RETN; rs1862513, rs3219177, rs3745367, rs3745368). Biochemical analysis included determination of circulating adipokines, C-reactive protein (CRP) levels, lipid profile, and markers of insulin resistance. Results: Compared to males, females presented higher circulating levels of insulin, adiponectin, IL-6, resistin, and leptin concentrations, but lower TNF-α levels. No statistically significant differences were found for genotype or allelic distributions between genders. In the whole sample population, adiponectin levels were influenced by ADIPOQ rs17300539 (c.-1138G>A; lower in subjects with GG genotype). When only males were considered, IL-1β, IL-6, and TNF-α levels were associated with ADIPOQ rs1501299 (c.214 + 62G>T; higher in GG subjects). TNF-α concentrations were modulated by TNF-αrs1800629 (c.-488G>A; lower in GG males), RETN rs1862513 (c.-216C>G; higher in CC subjects), and RETN rs3219177 (c.118 + 39C>T; higher in CC subjects). Leptin levels were influenced by IL-1β rs1143627 (c.-118C>T) presenting TT individuals' lower levels. Conclusions: Our data demonstrate that in pediatric obese patients, some adipokine gene SNPs have an association with circulating adipokine levels and lipid profile.
- Hepcidin-25 and treatment with erythropoiesis stimulating agents are independently related with erythropoiesis in chronic hemodialysis patientsPublication . Belo, Luís; Rocha, Susana; Valente, Maria João; Coimbra, Susana; Catarino, Cristina; Bronze-da-Rocha, Elsa; Carvalho, Márcia; Rocha-Pereira, Petronila; do Sameiro-Faria, Maria; Oliveira, José Gerardo; Madureira, José; Fernandes, João Carlos; Miranda, Vasco; Santos-Silva, Alice
- Machine learning in prediction of vulnerable or resilient end-stage renal disease patientsPublication . Pedroso, João Pedro; Valente, Maria João; Rocha, Susana; Coimbra, Susana; Catarino, Cristina; Rocha-Pereira, Petronila; Bronze-Da-Rocha, Elsa; Carvalho, Márcia; Oliveira, José Gerardo; Madureira, José; Fernandes, João Carlos; Do Sameiro-Faria, Maria; Miranda, Vasco; Belo, Luís; Santos-Silva, Alice
- Nutritional, chemical and antioxidant/pro-oxidant profiles of silverskin, a coffee roasting by-productPublication . Costa, Anabela S.G.; Alves, Rita C.; Ferreira da Vinha, Ana; Costa, Elísio; Nunes, M. Antónia; Almeida, Agostinho A.; Santos-Silva, Alice; Oliveira, M. Beatriz .P.P.Coffee silverskin (a coffee roasting by-product) contains high amounts of dietary fibre (49% insoluble and 7% soluble) and protein (19%). Potassium (∼5 g/100 g), magnesium (2 g/100 g) and calcium (0.6 g/100 g) are the major macrominerals. The vitamin E profile of silverskin comprises α-tocopherol, β-tocopherol, ɣ-tocopherol, δ-tocopherol, β-tocotrienol, ɣ-tocotrienol, and δ-tocotrienol. The fatty acid profile is mainly saturated (C16:0 and C22:0), but the total amount of fat is low (2.4%). Caffeine (1.25 g/100 g), chlorogenic acid (246 mg/100 g), and 5-hydroxymethylfurfural (5.68 mg/100 g) are also present in silverskin. Total phenolics and flavonoids are partially responsible for the in vitro antioxidant activity. Silverskin extracts protected erythrocytes from oxidative AAPH- and H2O2-induced hemolysis, but at high concentrations a pro-oxidant effect on erythrocyte morphology was observed.
- Protective activity of coffee silverskin extracts: evaluation on erythrocyte oxidative-induced hemolysisPublication . Ferreira da Vinha, Ana; Costa, Elísio; Costa, Anabela S.G.; Alves, Rita C.; Santos-Silva, Alice; Oliveira, M. Beatriz P.P.Natural antioxidants from industry by-products have been receiving increasing attention, having in view the sustainability of the processes. Coffee silverskin, a pellicule that covers the raw coffee bean, is a by-product of coffee roasting since it is detached during this procedure. Human erythrocytes are particularly useful in the evaluation of the antioxidant properties of bioactive compounds, since they are susceptible to endogenous oxidative damage due to their specific role as oxygen carriers. The aim of this work was to evaluate the capacity of natural antioxidants of coffee silverskin to protect erythrocytes from oxidative injury induced by the water soluble radical initiator 2,2-azobis (2 amidinopropane)dihydrochloride (AAPH) and by H2O2. The results showed that coffee silverskin extracts protected erythrocytes in a significant way from oxidative-AAPH and oxidative-H2O2 induced hemolysis. As far as we know, there are no investigations, which evaluate the protective activity of coffee silverskin extracts on human erythrocytes hemolysis.
- Valorização de frutos silvestres edíveis para consumo in naturaPublication . Ferreira da Vinha, Ana; Albuquerque, Carina; Costa, Anabela; Costa, Elísio; Manso, M. Conceição; Santos-Silva, Alice; Oliveira, M. BeatrizO consumo de frutos que contem compostos com ação antioxidante tem sido associado à redução do risco de incidência de doenças crónicas, causados por stresse oxidativo. A flora Portuguesa caracteriza-se por uma elevada biodiversidade de espécies vegetais, entre as quais os frutos de Crataegus monogyna Jacq. (pilrito), Rosa canina L. e Arbutus unedo L. (medronho), alvo do estudo que se apresenta. Procedeu-se à caracterização nutricional dos frutos inteiros e à avaliação dos compostos bioativos e da atividade antioxidante em extratos hidroalcoólicos. Os frutos apresentaram um perfil nutricional idêntico, caracterizado por teores elevados de água e hidratos de carbono e teores reduzidos de gordura. Quanto aos compostos bioativos determinados (compostos fenólicos, flavonoides e antocianinas) e a atividade antioxidante (DPPH• e FRAP) obtiveram-se teores promissores, com os flavonoides totais a mostrarem uma correlação positiva elevada com a atividade antioxidante. Foi, também, observada uma elevada atividade antioxidante dos extratos, através da avaliação da ação protetora de eritrócitos contra os danos oxidativos induzidos pelo oxidante AAPH. Os dados obtidos permitem pensar em novas aplicações destas matérias-primas, não só na indústria alimentar como também na indústria farmacêutica. A sua valorização pode ter um efeito muito positivo na sustentabilidade social e económica das zonas de produção destes frutos.