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- Atlas de apoio às aulas de Anatomia PatológicaPublication . Costa, Céu; Pereira, SofiaTodas as fotografias deste atlas são provenientes de lâminas histológicas realizadas no Laboratório de Anatomia Patológica da Universidade Fernando Pessoa, estando disponíveis para visualização pelos alunos durante as aulas.
- Oxidative stress, antioxidants and biomarkers: appreciation for analytical methods for health promotionPublication . Ferreira da Vinha, Ana; Silva, Carla Sousa e; Costa, CéuBecause of its extensive potential impact on human health, the determination of oxidative stress has been the main goal of study in several studies over the last few decades. The current work is a bibliographic review of the main methods of assessing oxidative stress in humans, taking into account the adverse effects it can do to individual quality of life and public health. To situate the theme, initially was described the concept of oxidative stress and the main diseases associated with it. Afterwards was presented the principal species of free radicals responsible for the oxidation, the antioxidant agents, enzymatic and non enzymatic , and the main bio marker of evaluation of oxidative stress. Regarding the analytical methods, it was proceeded by it's grouping according with the analyte in cause. In this regard of the reactive species of oxygen (ROS) and nitrogen (RNS), are presented the principal fluorescent and chemiluminescent probes, more concretely lucigenin, dihydroethidium DHE, and MitoSox, all characterized of the measurement of the superoxide, just like the relative to the determination of hydrogen peroxide as 2', 7'- dichlorodihydrofluorescein diacetate (DCFH-DA), boronate and Amplex Red. About the RNS, are shown the 4,5-diaminofluorescein (DAF2-DA) and the dihydroethidium fluorescent probes as a method of determination of the level of NO and peroxynitrite, respectively. For biomarkers, the current work, presents the analysis methods dedicated to the detection of F2-isoprostanes, lipid hydroperoxides and malondialdehyde (MDA) associated with lipid peroxidation. Chromatographic methods, hyphenated with chemiluminescence spectroscopy, fluorescence, and mass spectrometry (MS/MS), and electrochemical detection (ECD) is the most frequently used along with the enzymatic immunoassays (ELISA). With regard to antioxidants, diversity methodologies is most evident, although the methods overridden by liquid chromatography.
- A delecção do gene TP53 e a acumulação do p53 (AB-240) são importantes na determinação do prognóstico no carcinoma urotelial da bexigaPublication . Pereira, Sofia; Costa, Céu; Lameiras, Catarina; Amaro, Teresina; Bento, Maria; Koch, Alberto; Santos, LúcioTP53 gene deletions or mutations were related to more aggressiveness in invasive and in situ bladder tumors. However, its prognostic value in the superficial ones is not well defined. Sixty-two cases with low-grade superficial urothelial cell carcinomas (uccs) were studied. twenty-two suffered recurrence and 10 of these progressed. FiSH for TP53 detection and immunohistochemistry for p53 with both Do7 and Ab-240 antibodies were used. TP53 aberrations were suggestively related to p53 (Ab-240) positive immunoexpression (p=0.06). the 5-year recurrence-free survival was significantly lower (p= 0.002) in cases with TP53 monosomic deletion and these had a much lower 5-year progression-free survival (p= 0.075). the p53 (Ab-240) negative cases showed a significantly better outcome (p=0.04). the superficial papillary uccs with TP53 monosomic deletion or p53 Ab-240 positive immunoexpression had a poor outcome, being these surrogate biologic factors of poor prognosis. As delecções ou mutações do gene TP53 têm sido relacionadas com a maior agressividade dos carcinomas da bexiga in situ ou invasores. no entanto, o seu valor prognóstico nos tumores superficiais ainda não está definido. Foram estudados 62 tumores da bexiga superficiais e de baixo grau. Vinte e dois sofreram recorrência e 10 destes progrediram. utilizou-se o FiSH para a detecção do gene TP53 e a imunohistoquímica para a proteína p53 com os anticorpos Do7 e Ab-240. As alterações do gene TP53 relacionaram-se com a imunoexpressão positiva da p53 (Ab-240; p=0,06). A sobrevivência livre de recorrência aos 5 anos foi significativamente menor (p=0,002) nos doentes cujos tumores tinham delecção do gene TP53, tendo também uma menor sobrevivência livre de progressão (p=0,075). os doentes cujos tumores eram negativos para a p53 (Ab-240) mostraram resultados significativamente melhores (p=0,04). os doentes cujos tumores apresentavam delecção do gene TP53 ou imunoexpressão para a p53 com o anticorpo Ab-240, mostraram piores resultados, podendo estes ser potenciais marcadores biológicos de pior prognóstico.