CEBIMED - Papers/ Artigos Científicos
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Browsing CEBIMED - Papers/ Artigos Científicos by Author "Andrade, Paula B."
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- Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruitsPublication . Ferreira da Vinha, Ana; Silva, Branca M.; Andrade, Paula B.; Seabra, Rosa M.; Pereira, José A.; Oliveira, M. Beatriz P.P.A new HPLC=DAD methodology for separating nine phenolic compounds is described. This methodology is applied to the definition of qualitative and quantitative profiles of three Portuguese olive fruit cultivars (Cobranc¸osa, Madural and Verdeal). Two different extraction methods were needed for the complete definition of their profiles, one of them including a Sep-pack C18 cleaning step.The chromatographic separation was achieved using a Spherisorb ODS2 (25.060.46 cm; 5 mm, particle size) column.The solvent system used was a gradient of water-formic acid (19:1) and methanol, with a flow rate of 0.9 mL=min. The detection limit values for phenolic compounds were between 0.04 and 4.32 mg=mL and the method was precise. As ageneral rule, the recovery values were high. This technique can also be useful in the discrimination of Portuguese olive fruit cultivars.
- Phenolic profiles of portuguese olive fruits (Olea europaea L.): influences of cultivar and geographical originPublication . Ferreira da Vinha, Ana; Ferreres, Federico; Silva, Branca M.; Valentão, Patrícia; Gonçalves, Ana; Pereira, José; Oliveira, M. Beatriz P.P.; Seabra, Rosa M.; Andrade, Paula B.The phenolic compounds present in 29 samples of olive fruits were analysed by reversed-phase HPLC/DAD and/or HPLC-DAD/ ESI-MS/MS. All samples were collected during the normal picking period for olive oil production, in north and central Portugal, and were obtained from 18 different olive cultivars. Two different extraction methods were necessary for the complete quantification of phenolic compounds, a methanolic extraction and an extraction which included a solid-phase extraction (SPE) cleaning step. The analyses showed that all samples presented a similar profile, which included at least six identified phenolic compounds: hydroxytyrosol, luteolin 7-O-glucoside, oleuropein, rutin, apigenin 7-O-glucoside and luteolin. Several samples also contained 5-O- caffeoylquinic acid, verbascoside, quercetin 3-O-rhamnoside, cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside. In all samples, hydroxytyrosol and oleuropein were the major compounds identified while, in general, rutin and luteolin 7-O- glucoside were the two main flavonoids. The influences of maturation index, nature of the cultivar and geographical origin are discussed.