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Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruits
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Resumo(s)
A new HPLC=DAD methodology for separating nine phenolic compounds is described. This methodology is applied to the definition of qualitative and quantitative profiles of three Portuguese olive fruit cultivars (Cobranc¸osa, Madural and Verdeal). Two different extraction methods were needed for the complete definition of their profiles, one of them including a Sep-pack C18 cleaning step.The chromatographic separation was achieved using a Spherisorb ODS2 (25.060.46 cm; 5 mm, particle size) column.The solvent system used was a gradient of water-formic acid (19:1) and methanol, with a flow rate of 0.9 mL=min.
The detection limit values for phenolic compounds were between 0.04 and 4.32 mg=mL and the method was precise. As ageneral rule, the recovery values were high. This technique can also be useful in the discrimination of Portuguese olive fruit cultivars.
Descrição
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Contexto Educativo
Citação
Ana F. Vinha , Branca M. Silva , Paula B. Andrade , Rosa M. Seabra , José A. Pereira & M. Beatriz Oliveira (2002) Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruits. Journal of Liquid Chromatography & Related Technologies, 25:1, 151-160, DOI: 10.1081/JLC-100108546
Editora
Taylor & Francis