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Publication
Analysis of microbial contamination of gutta-percha points commonly used in clinical practice: a practical approach
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Authors
Advisor(s)
Abstract(s)
Objectivos
Avaliar a contaminação bacteriana dos cones de Gutta-Percha utilizados rotineiramente na prática clínica e a eficácia de um Protocolo de Desinfecção “Chairside”.
Métodos
Cones de Gutta-Percha (n240) nos tamanhos A,B,C,D,K15,K20,K25,K30,K35,K40,F1,F2,F3 (Dentsply®, Proclinic®, ProTaper® e R&S®) foram recolhidos, aleatoriamente, de embalagens comerciais abertas em uso e, de imediato, adicionados ao Meio Fluído de Tioglicolato e incubados, a 37ºC, durante 21dias para avaliação da presença ou ausência de turvação. Para testar a eficácia de um Protocolo de Desinfecção, os cones de Gutta-Percha detectados como contaminados foram imersos durante 1minuto em 10mL de Hipoclorito de Sódio a 5,25%, seguidos de 5 minutos em 10mL de solução detergente (3% Tween 80 e 5% de Tiossulfato de Sódio) e a lavagem final foi feita com 10mL de Água Destilada Estéril, tendo sido novamente incubados nas condições descritas anteriormente.. Os dados foram analisados pelo teste do Qui-Quadrado com nível de significância de 5%.
Resultados
Observou-se crescimento bacteriano em 22,9% das amostras (Dentsply® e R&S® apresentaram o maior número de contaminados 47,3% cada). O calibre mais contaminado foi o K30 (16,4%), mas todos os cones de calibre D mostraram contaminação microbiana. O Protocolo de Desinfecção “Chairside” mostrou-se eficaz em 76,4% dos casos.
Conclusões
Um pequeno número de cones de Gutta-Percha em uso clínico mostrou contaminação microbiana, inclusive após o Protocolo de Desinfecção “Chairside”, que, contudo, provou ser consideravelmente eficaz. Não se observou nenhuma diferença estatisticamente significativa entre as marcas comerciais em teste. É necessário dar particular atenção ao controlo da contaminação nosocomial durante todas as fases do Tratamento Endodontico Não-Cirúrgico de forma a melhor garantir o seu sucesso.
Aim To evaluate the bacterial contamination of Gutta-Percha points routinely used in clinical practice and the efficacy of a “Chairside” Disinfection Protocol. Methodology Gutta-Percha points (n240), in sizes A,B,C,D,K15,K20,K25,K30,K35,K40,F1,F2,F3 (Dentsply®, Proclinic®, ProTaper® and R&S®), were randomly sampled from open commercial packages in use. These were added directly to Fluid Thioglycolate Medium and incubated, at 37ºC, for 21days. During this period, the presence/absence of turbidity was evaluated. To evaluate the efficacy of a “Chairside” Disinfection Protocol, all detected contaminated Gutta-Percha points were immersed for 1minute in 10mL of 5,25% sodium hypochlorite, followed by 5minutes in 10mL of detergent solution (3% Tween 80 and 5% Sodium Thiosulfate) and a final rinse with 10mL of Sterile Distilled Water and incubated, again, as described before. Data were analysed by the chi-square test at 5% significance level. Results Bacterial growth was observed in the 22,9% of samples (Dentsply® and R&S® showed the highest number of contaminated 47,3% each). The most contaminated gauge was K30 (16.4%), but, all D gauge were found to be contaminated. The “Chairside” Disinfection Protocol resulted effective in 76,4% of cases. Conclusions A small number of Gutta-Percha points in clinical use harboured microorganisms, including after the “Chairside” Disinfection Protocol that, anyway, proved to be remarkably effective. No significant difference was observed between the commercials brands in test. Awareness in nosocomial contamination control should always be performed during all stages of Non-Surgical Root Canal Treatment to better ensure its success.
Aim To evaluate the bacterial contamination of Gutta-Percha points routinely used in clinical practice and the efficacy of a “Chairside” Disinfection Protocol. Methodology Gutta-Percha points (n240), in sizes A,B,C,D,K15,K20,K25,K30,K35,K40,F1,F2,F3 (Dentsply®, Proclinic®, ProTaper® and R&S®), were randomly sampled from open commercial packages in use. These were added directly to Fluid Thioglycolate Medium and incubated, at 37ºC, for 21days. During this period, the presence/absence of turbidity was evaluated. To evaluate the efficacy of a “Chairside” Disinfection Protocol, all detected contaminated Gutta-Percha points were immersed for 1minute in 10mL of 5,25% sodium hypochlorite, followed by 5minutes in 10mL of detergent solution (3% Tween 80 and 5% Sodium Thiosulfate) and a final rinse with 10mL of Sterile Distilled Water and incubated, again, as described before. Data were analysed by the chi-square test at 5% significance level. Results Bacterial growth was observed in the 22,9% of samples (Dentsply® and R&S® showed the highest number of contaminated 47,3% each). The most contaminated gauge was K30 (16.4%), but, all D gauge were found to be contaminated. The “Chairside” Disinfection Protocol resulted effective in 76,4% of cases. Conclusions A small number of Gutta-Percha points in clinical use harboured microorganisms, including after the “Chairside” Disinfection Protocol that, anyway, proved to be remarkably effective. No significant difference was observed between the commercials brands in test. Awareness in nosocomial contamination control should always be performed during all stages of Non-Surgical Root Canal Treatment to better ensure its success.
Description
Keywords
Endodontic treatment Root canal filling Guta-percha points Contamination Disinfection protocol Secondary endodontic infection