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Prevalência, suscetibilidade aos antimicrobianos e avaliação de resistência por testes imunocromatográficos dos agentes de infecções urinárias comunitárias
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| DM_33245 | 2.8 MB | Adobe PDF |
Authors
Advisor(s)
Abstract(s)
Mostra-se de grande importância um maior conhecimento acerca da temática da
resistência antimicrobiana naqueles casos que envolvem infecção do trato urinário.
Ainda, a motivação encontra-se na observação de que estes tipos de infecção são os
mais comumente encontrados no cotidiano do atendimento clínico geral, sendo de
grande relevância que se conheçam formas adequadas de se identificar cepas resistentes,
de forma rápida, segura e eficiente e, assim, possibilitar um tratamento mais efetivo
diante destes casos.
Com o presente estudo pretende-se analisar o emprego de testes
imunocromatográficos e suas vantagens na identificação da prevalência e
suscetibilidade dos agentes etiológicos causadores de infecção urinária comunitária. Foi
elaborado a partir da análise de urinoculturas realizadas pelo laboratório J. L.
PATOLOGIA CLÍNICA, no período de 01 de julho a 31 de dezembro de 2019. Todas as
amostras são oriundas de pacientes das comunidades de Marataízes e Itapemirim,
municípios limítrofes, que foram recebidos através dos postos de coleta do laboratório
instalados nos mesmos, após consultas médicas nas clínicas e postos de saúde da região.
As espécimes de urina foram semeadas em ágar Cps cromogenico, (BioMérieux, Marcy
L´Étoile, France), com alça calibrada 1/1000, sendo feito a incubação das placas a 37
graus por 24 horas, considerou-se culturas positivas contagem de colônias superior a
100.000 ufc/ ml de urina.
Após o crescimento em ágar cps cromogênico, as amostras foram ressemeadas
em ágar sangue, (BioMérieux, Marcy L´Étoile, France), por 12 horas a 37 graus celsius,
com a finalidade de maximizar suas características metabólicas neste meio rico em
nutrientes, para posterior realização dos testes cromatográficos e também para o TSA.
Foram analisadas 2.640 urinoculturas, sendo que 766 amostras totalizando 29,01%
foram consideradas positivas. Conclui-se que o teste rápido imunocromatográfico é de
grande importância para a identificação de cepas resistentes a antibióticos de interesse
em saúde pública, representando uma opção simples, segura e eficaz, cada vez mais
utilizada no cotidiano clínico, especialmente a nível hospitalar.
Greater knowledge about the subject of antimicrobial resistance is shown to be of great importance in those cases involving urinary tract infection. Still, the motivation is found in the observation that these types of infections are the most commonly found in the routine of general clinical care, and it is of great relevance that adequate ways of identifying resistant strains are known, quickly, safely and efficiently and , thus, enabling a more effective treatment in these cases. This study aims to analyze the use of immunochromatographic tests and their advantages in identifying the prevalence and susceptibility of the etiologic agents that cause community urinary infection. It was prepared from the analysis of urine cultures performed by the laboratory JL PATOLOGIA CLÍNICA, from July 1 to December 31, 2019. All samples come from patients from the communities of Marataízes and Itapemirim, bordering municipalities, who they were received through the laboratory's collection points installed there, after medical consultations in the clinics and health posts in the region. The urine specimens were seeded on chromogenic Cps agar, (BioMérieux, Marcy L´Étoile, France), with a 1/1000 calibrated loop, and the plates were incubated at 37 degrees for 24 hours, positive cultures were counted as colony count greater than 100,000 cfu / ml of urine. After growing on chromogenic cps agar, the samples were reseeded in blood agar, (BioMérieux, Marcy L´Étoile, France), for 12 hours at 37 degrees celsius, in order to maximize their metabolic characteristics in this nutrient-rich medium, for subsequent performance of the chromatographic tests and also for the TSA. 2.640 urine cultures were analyzed, with 766 samples totaling 29.01% were considered positive. It is concluded that the rapid immunochromatographic test is of great importance for the identification of strains resistant to antibiotics of interest in public health, representing a simple, safe and effective option, increasingly used in clinical practice, especially at the hospital level.
Greater knowledge about the subject of antimicrobial resistance is shown to be of great importance in those cases involving urinary tract infection. Still, the motivation is found in the observation that these types of infections are the most commonly found in the routine of general clinical care, and it is of great relevance that adequate ways of identifying resistant strains are known, quickly, safely and efficiently and , thus, enabling a more effective treatment in these cases. This study aims to analyze the use of immunochromatographic tests and their advantages in identifying the prevalence and susceptibility of the etiologic agents that cause community urinary infection. It was prepared from the analysis of urine cultures performed by the laboratory JL PATOLOGIA CLÍNICA, from July 1 to December 31, 2019. All samples come from patients from the communities of Marataízes and Itapemirim, bordering municipalities, who they were received through the laboratory's collection points installed there, after medical consultations in the clinics and health posts in the region. The urine specimens were seeded on chromogenic Cps agar, (BioMérieux, Marcy L´Étoile, France), with a 1/1000 calibrated loop, and the plates were incubated at 37 degrees for 24 hours, positive cultures were counted as colony count greater than 100,000 cfu / ml of urine. After growing on chromogenic cps agar, the samples were reseeded in blood agar, (BioMérieux, Marcy L´Étoile, France), for 12 hours at 37 degrees celsius, in order to maximize their metabolic characteristics in this nutrient-rich medium, for subsequent performance of the chromatographic tests and also for the TSA. 2.640 urine cultures were analyzed, with 766 samples totaling 29.01% were considered positive. It is concluded that the rapid immunochromatographic test is of great importance for the identification of strains resistant to antibiotics of interest in public health, representing a simple, safe and effective option, increasingly used in clinical practice, especially at the hospital level.
Description
Keywords
Resistência microbiana Resistência a antibióticos Teste imunocromatográfico Microbial resistance Antibiotic resistance Immunochromatographic test