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Avaliação da atividade antioxidante de plantas medicinais africanas
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Abstract(s)
Objetivo: Este trabalho teve como objetivo avaliar o teor de compostos
bioativos (fenólicos e flavonoides totais), bem como estudar a atividade antioxidante de
duas espécies botânicas (Moringa oleifera e Ruta chalepensis) cultivadas e utilizadas na
medicina tradicional de Cabo Verde.
Metodologia: A determinação dos compostos fenólicos totais e flavonoides
totais foi efetuada recorrendo a técnicas espetofotométricas seguindo-se a metodologia
previamente validada por Vinha et al.. Quanto à avaliação da atividade antioxidante
utilizaram-se dois métodos espetrofotométricos complementares: inibição do radical
livre DPPH• (2,2-difenil-1-picrilhidrazilo) e o poder antioxidante por redução do ião
férrico (FRAP). Em todos os métodos os resultados foram obtidos a partir de ensaios
realizados em triplicado.
Resultados: O teor de compostos fenólicos e flavonoides totais foi
significativamente superior nos extratos aquosos de Ruta chalepensis (12,01 mg/ 100g
e 4,93 mg/ 100g, respetivamente) face aos observados na Moringa oleifera (6,76 mg/
100g e 0,98 mg/ 100g, respetivamente). Quanto à atividade antioxidante, a Ruta
chalepensis obteve um valor inferior ao da Moringa oleifera (80,4 μg/ ml e 300 μg/ ml,
respetivamente), no que concerne ao método DPPH•. O mesmo não se observou no
método FRAP, dado que a M. oleifera apresentou um poder redutor superior ao da
Ruta chalepensis, 1900 μg/ ml face a 1093 μg/ ml, respetivamente.
Conclusão: O teor de compostos bioativos assim como a atividade antioxidante
foi superior nos extratos aquosos de Ruta chalepensis. Os compostos bioativos
presentes nas plantas medicinais possuem substâncias úteis para o tratamento ou
alívio de sintomas de variadas doenças. No entanto, são necessários mais estudos que
comprovem a eficácia das mesmas.
Objective: This work aimed to evaluate the content of bioactive compounds (total phenolic and total flavonoids), as well as to study the antioxidant activity of two botanical species (Moringa oleifera and Ruta chalepensis) cultivated and used in traditional medicine in Cape Verde. Methodology: The determination of total phenolic compounds and total flavonoids was carried out using spectrophotometric techniques following the methodology previously validated by Vinha et al.. Regarding the evaluation of antioxidant activity, two complementary spectrophotometric techniques were used: free radical inhibition DPPH• (2,2-diphenyl-1-picrylhydrazyl) and the antioxidant power by ferric ion reduction (FRAP). In all determinations the results were obtained from triplicate assays. Results: The content of phenolic compounds and total flavonoids was significantly higher in aqueous extracts of Ruta chalepensis (12.01 mg/ 100g and 4.93 mg/ 100g, respectively) compared to Moringa oleifera (6.76 mg/ 100g and 0.98 mg/ 100g, respectively). Regarding antioxidant activity, Ruta chalepensis obtained a lower value than Moringa oleifera (80.4 μg/ ml and 300 μg/ ml, respectively), regarding the DPPH• method. The same was not observed in the FRAP method, as M. oleifera had a lower reducing power than Ruta chalepensis, 1900 μg/ ml compared to 1093 μg/ ml, respectively. Conclusion: The content of bioactive compounds as well as the antioxidant activity was higher in the aqueous extracts of Ruta chalepensis. The bioactive compounds present in medicinal plants have substances useful for treating or relieving symptoms of various diseases. However, further studies are needed to prove their effectiveness.
Objective: This work aimed to evaluate the content of bioactive compounds (total phenolic and total flavonoids), as well as to study the antioxidant activity of two botanical species (Moringa oleifera and Ruta chalepensis) cultivated and used in traditional medicine in Cape Verde. Methodology: The determination of total phenolic compounds and total flavonoids was carried out using spectrophotometric techniques following the methodology previously validated by Vinha et al.. Regarding the evaluation of antioxidant activity, two complementary spectrophotometric techniques were used: free radical inhibition DPPH• (2,2-diphenyl-1-picrylhydrazyl) and the antioxidant power by ferric ion reduction (FRAP). In all determinations the results were obtained from triplicate assays. Results: The content of phenolic compounds and total flavonoids was significantly higher in aqueous extracts of Ruta chalepensis (12.01 mg/ 100g and 4.93 mg/ 100g, respectively) compared to Moringa oleifera (6.76 mg/ 100g and 0.98 mg/ 100g, respectively). Regarding antioxidant activity, Ruta chalepensis obtained a lower value than Moringa oleifera (80.4 μg/ ml and 300 μg/ ml, respectively), regarding the DPPH• method. The same was not observed in the FRAP method, as M. oleifera had a lower reducing power than Ruta chalepensis, 1900 μg/ ml compared to 1093 μg/ ml, respectively. Conclusion: The content of bioactive compounds as well as the antioxidant activity was higher in the aqueous extracts of Ruta chalepensis. The bioactive compounds present in medicinal plants have substances useful for treating or relieving symptoms of various diseases. However, further studies are needed to prove their effectiveness.
Description
Trabalho Complementar apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Licenciada em Ciências da Nutrição
Keywords
Moringa oleifera Ruta chalepensis Compostos bioativos Atividades biológicas Medicina tradicional Moringa oleifera Ruta chalepensis Bioactive compounds Biological properties Traditional medicine