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- Mental health and alcohol consumption among university students in the post-pandemic context: an exploratory cross-sectional study in PortugalPublication . Moreira, Teresa; Guimarães, Maria Inês; Silveira, Augusta; Loibl, Beatriz; Guedes Lopes, Beatriz; Alves Ribeiro Ferraz, Hugo Filipe; Castro, Inês; Mira de Almeida, Sofia; Lopes Cardoso, Inês; Pereira Rodrigues, Sandra Beatriz; Lima, Andreia Maria NovoIntroduction: The COVID-19 pandemic had significant effects on mental health and lifestyle behaviours, especially among university students who experienced academic disruptions, social isolation, and fewer social interactions. Alcohol consumption has long been part of student culture. Still, the influence of post-pandemic academic reintegration on drinking patterns and psychological distress remains relatively unexplored, particularly in countries like Portugal, where student traditions heavily shape consumption habits. This study aimed to describe the prevalence of alcohol consumption, depression, anxiety, and stress in a sample of Portuguese university students during the post-pandemic academic period, and to explore associations with sociodemographic variables. Methods: A cross-sectional study was conducted in November 2021 with 90 students from a private higher education institution in northern Portugal. Data were collected via an online questionnaire including sociodemographic information, the Alcohol Use Disorders Identification Test (AUDIT), and the Depression, Anxiety and Stress Scale (DASS-21). Result: The majority of the participants were not at risk of alcohol addiction (95.3%). In total, 15.1% of students reported anxiety symptoms ranging from severe to extremely severe. A binomial logistic regression was performed to ascertain the effects of being away from home and psychological distress (DASS-42 score), on the likelihood that participants were at risk of alcohol addiction (Level 3 and 4 in the AUDIT scale). The logistic regression model was statistically significant, χ2(2) = 9.20, p = 0.010. Living away from home was associated with a substantially lower likelihood of high-risk status (B = −2.79, p = 0.034), corresponding to an odds ratio of 0.06, indicating a strong protective effect. DASS-42 total score was positively associated with high-risk status (B = 0.04, p = 0.039), such that higher psychological distress increased the odds of being classified as high risk. Conclusions: The findings reveal a low prevalence of alcohol risk but heightened symptoms of anxiety, depression, and stress. Psychological distress notably increases the likelihood of hazardous alcohol use, emphasising the importance of targeted mental health and alcohol-use interventions among university students.
- Comparison of spectroscopic and molecular methods for estimating age and sex for medico-legal purposes: protocol for a systematic reviewPublication . Lopes Cardoso, Inês; Loibl, Beatriz; Rodrigues, Carolina; Moreira, Teresa; Guimarães, Maria InêsIntroduction: In forensic contexts, accurate estimation of age and sex is fundamental for the identification of human remains. Spectroscopic methods, offering non-destructive and precise data insights, have emerged as promising alternatives/complements to traditional morphological and genetic techniques. Objective: This protocol outlines a systematic review aimed at evaluating the effectiveness of spectroscopic and molecular methods for estimating age and/or sex based on dental samples in medicolegal settings. Methodology: This review will be conducted in accordance with the PRISMA 2020 guidelines. Databases to be searched include PubMed, Web of Science, ScienceDirect, LILACS (via BVS), Cochrane Library (Protocols), MEDLINE (via EBSCOhost), Academic Search Complete (via EBSCOhost), CINAHL (via EBSCOhost) and grey literature. The eligibility criteria follow the PICOS framework. Risk of bias will be assessed using the QUIN tool for in vitro studies. Data synthesis will involve both quantitative and qualitative approaches. The protocol of this review has been registered in PROSPERO (CRD420250650858). Results: This review is expected to identify a range of spectroscopic techniques applied to dental samples for age and sex estimation, including mass spectrometry, Raman spectroscopy, FTIR spectroscopy, and X-ray fluorescence, as well as molecular methods such as amelogenin analysis, DNA methylation, and telomere length assessment. The evidence is anticipated to vary regarding study design, sample characteristics, analytical protocols, and reported outcome measures. The review will synthesize the available evidence on the accuracy, applicability, and methodological characteristics of these approaches within medico-legal contexts. Conclusion: This systematic review will provide a comprehensive overview of the available evidence regarding the use of spectroscopic and molecular methods for age and sex estimation from dental samples. By identifying the methodologies currently employed, their reported outcomes, and existing research gaps, the review may contribute to future research directions and support evidence-based decision-making in forensic and medico-legal practice, particularly in contexts where non-destructive analytical approaches are desirable.
