Repository logo
 

FCS (DCF) - Artigos em Atas de ConferĂȘncias Internacionais

Permanent URI for this collection

http://hdl.handle.net/10284/3056

Browse

Recent Submissions

Now showing 1 - 1 of 1
  • A biophysical approach to formulation development: drug-plasma protein interaction
    Publication . Fernandes, Eduarda; Soares, Telma; Oliveira, M.E.C.D. Real; Lopes, Carla Martins; LĂșcio, Marlene
    In this work, the binding constant of ACV to human serum albumin was determined by binding isotherms. Nonlinear leastsquares best fit to intrinsic fluorescence quenching effect of the drug was used to determine the association constants between drug and the serum protein at one affinity binding site (Kb=1.79x103 M-1). A red shift of the fluorescent spectra confirms association. Thermodynamic parameters for the binding indicated that electrostatic interactions are predominantly involved in the binding of this drug to human serum albumin. This was further confirmed by electrophoretic and dynamic light scattering, where a progressive charge neutralization of the protein was followed by an increase of the size of albumin-drug complex.
    2018-06-27Conference object Restricted access Show more