FCS - Comunicações em Conferências Internacionais
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Browsing FCS - Comunicações em Conferências Internacionais by Author "Araújo, Ana Margarida"
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- Early pathways of toxicity triggered by 3,4-methylenedioxypyrovalerone unveiled by in vivo toxicometabolomicsPublication . Araújo, Ana Margarida; Carvalho, Márcia; Costa, Vera Marisa; Duarte, José Alberto; Dinis-Oliveira, Ricardo; Bastos, Maria de Lourdes; Guedes de Pinho, Paula; Carvalho, Félix
- Effect of subtoxic and toxic concentrations of galactosamine in the metabolome of primary mouse hepatocytesPublication . Araújo, Ana Margarida; Lima, Ana Rita; de Lourdes Bastos, Maria; Carvalho, Félix; Carvalho, Márcia; de Pinho, Paula Guedes
- In vitro volatile exometabolome signature of clear cell renal cell carcinomaPublication . Amaro, Filipa; Pinto, Joana; Rocha, Sílvia; Araújo, Ana Margarida; Gonçalves, Vera Miranda; Jerónimo, Carmen; Henrique, Rui; Bastos, Maria de Lourdes; Carvalho, Márcia; Guedes de Pinho, Paula
- A new strategy for exploring the role of hyperthermia in MDMA-induced toxicity in primary mouse hepatic cells using GC-MS-based metabolomicsPublication . Araújo, Ana Margarida; Enea, Maria; Fernandes, Eduarda; Bastos, Maria de Lourdes; Carvalho, Félix; Guedes de Pinho, Paula; Carvalho, Márcia
- Toxicometabolomic insights into the mechanisms of hepatotoxicity triggered by amphetaminic drugsPublication . Araújo, Ana Margarida; Carvalho, Márcia; Fernandes, Eduarda; Bastos, Maria de Lourdes; Carvalho, Félix; Guedes de Pinho, Paula
- Volatile exometabolome profiling of human renal cell carcinoma cell lines for biomarker discoveryPublication . Rocha, Sílvia; Amaro, Filipa; Pinto, Joana; Araújo, Ana Margarida; Miranda-Gonçalves, Vera; Jerónimo, Carmen; Henrique, Rui; Bastos, Maria de Lourdes; Carvalho, Márcia; Guedes de Pinho, PaulaRenal Cell Carcinoma (RCC) constitutes approximately 90-95% of all kidney neoplasms and is the second most lethal urological cancer. Current diagnostic techniques rely on imaging techniques and an invasive procedure (biopsy) is always required for histopathologic confirmation of malignancy. For these reasons, the identification of accurate biomarkers to develop faster, less invasive and more sophisticated diagnostic techniques is of utmost importance. Metabolomics has been widely applied in cancer biomarker discovery arising from the fact that cancer cells are metabolically reprogrammed to control the energy required by the rapid growth and development of the tumor, producing a specific “metabolic signature”. Aim: To evaluate the potential of volatile organic compounds (VOCs) and volatile carbonyl compounds (VCCs) to discriminate the exometabolome of RCC from non-tumoral cell lines, and two different histological subtypes (clear cell and papillary RCC) in both metastatic and non-metastatic stages. Methods: Headspace-solid phase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS)-based metabolomics was applied for the volatile profiling of culture medium of five different tumoral cell lines, namely three clear cell (769-P, 786-O and Caki-1) and two papillary RCC (Caki-2 and ACHN), and one non-tumoral cell line (HK-2). Results: Multivariate and univariate analysis unveiled a panel of metabolites responsible for the discrimination between each RCC cell line vs. non-tumoral cells, metastatic vs. non-metastatic and clear cell vs. papillary RCC cell lines, mostly belonging to alcohols, aldehydes, alkanes and ketones classes. Some metabolites were found similarly altered for all RCC cell lines compared to the non-tumoral, while others unveiled specificity for each RCC cell line. Discussion/Conclusion: The volatile exometabolome signature of RCC cells can provide candidate biomarkers for the development of a volatile sensor-based approach for non-invasive diagnosis of RCC in urine.