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Permeability assay and inflammatory marker quantification of lactoferrin functionalized lipid nanoparticles intended for brain delivery

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dc.contributor.authorTeixeira, Maria Inês
dc.contributor.authorLopes, Carla Martins
dc.contributor.authorReguengo, Henrique
dc.contributor.authorOliveira, José Carlos
dc.contributor.authorSilva, Ana Margarida
dc.contributor.authorRodrigues, Francisca
dc.contributor.authorAmaral, Maria Helena
dc.contributor.authorCosta, Paulo C.
dc.date.accessioned2024-01-31T17:01:52Z
dc.date.available2024-01-31T17:01:52Z
dc.date.issued2022
dc.description.abstractAmyotrophic lateral sclerosis (ALS) is a neurodegenerative disease with a survival rate of 3 to 5 years from the onset of symptoms. ALS treatment is compromised by the existence of the blood-brain barrier (BBB), which restricts the access of promising biopharmaceutics to the brain, including riluzole, a drug commonly used to treat ALS. To circumvent the BBB and improve the drug brain targeting, nanosystems such as lipid nanoparticles can be employed. In this work, the permeation of nanostructured lipid carriers (NLC) loaded with riluzole and functionalized with a specific ligand – lactoferrin – was assessed in an in vitro BBB model (hCMEC/D3 cell line). Moreover, the effect of the NLC on the production and secretion of the pro-inflammatory cytokine human interleukin 1 alpha (IL-1a) by the cells was also quantified. The permeability studies across the hCMEC/D3 cell monolayers showed that free riluzole penetrated the BBB more than the riluzole-loaded NLC, which was also consistent with the results from the ELISA kit, with the free drug eliciting a higher IL-1a production. Despite these findings, the developed nanocarriers possessed good biocompatibility and stability, and could, therefore, be considered suitable for brain applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTeixeira, M.I.; Lopes, C.M.; Reguengo, H.; Oliveira, J.C.; Silva, A.M.; Rodrigues, F.; Amaral, M.H. and Costa, P.C. (2022). Permeability assay and inflammatory marker quantification of lactoferrin functionalized lipid nanoparticles intended for brain delivery. Proceedings of the Nanotech International Conference (Nanotech France 2022), 15th – 17th June, Paris, France.pt_PT
dc.identifier.doihttps://www.setcor.org/files/papers/1665343198_Proceedings-Nanotech-France-2022.pdfpt_PT
dc.identifier.urihttp://hdl.handle.net/10284/12637
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectBlood-brain barrier (BBB)pt_PT
dc.subjectBrain deliverypt_PT
dc.subjectNeurodegenerationpt_PT
dc.subjectAmyotrophic lateral sclerosis (ALS)pt_PT
dc.subjectFunctionalized lipid nanoparticlespt_PT
dc.subjectNanostructured lipid carriers (NLC)pt_PT
dc.subjectRiluzolept_PT
dc.subjectLactoferrinpt_PT
dc.titlePermeability assay and inflammatory marker quantification of lactoferrin functionalized lipid nanoparticles intended for brain deliverypt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceParispt_PT
oaire.citation.endPage5pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleThe Nanotech France 2022 International Conferencept_PT
person.familyNameLopes
person.givenNameCarla
person.identifier.ciencia-id901D-160C-633E
person.identifier.orcid0000-0001-5080-032X
person.identifier.ridM-4689-2016
person.identifier.scopus-author-id26649517700
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationd2a48cfe-9258-4916-99be-bbcb710f6605
relation.isAuthorOfPublication.latestForDiscoveryd2a48cfe-9258-4916-99be-bbcb710f6605

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