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Staphylococcus aureusandEscherichia colidual-species biofilms on nanohydroxyapatite loaded with CHX or ZnO nanoparticles

2017Journal article Open access
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dc.contributor.authorBarros, Joana
dc.contributor.authorGrenho, Liliana
dc.contributor.authorFontenente, Sílvia
dc.contributor.authorManuel, Cândida M.
dc.contributor.authorNunes, Olga C.
dc.contributor.authorMelo, Luís F.
dc.contributor.authorMonteiro, Fernando J.
dc.contributor.authorFerraz, Maria Pia
dc.date.accessioned2019-09-12T14:29:05Z
dc.date.available2025-01-01T01:30:18Z
dc.date.issued2017
dc.description.abstractImplant-associated infections are caused by surface-adhering microorganisms persisting as biofilms, resistant to host defense and antimicrobial agents. Given the limited efficacy of traditional antibiotics, novel strategies may rely on the prevention of such infections through the design of new biomaterials. In this work, two antimicrobial agents applied to nanohydroxyapatite materials-namely, chlorhexidine digluconate (CHX) and zinc oxide (ZnO) nanoparticles-were compared concerning their ability to avoid single- or dual-species biofilms of Staphylococcus aureus and Escherichia coli. The resulting biofilms were quantified by the enumeration of colony-forming units and examined by confocal microscopy using both Live/Dead staining and bacterial-specific fluorescent in situ hybridization. The sessile population arrangement was also observed by scanning electron microscopy. Both biomaterials showed to be effective in impairing bacterial adhesion and proliferation for either single- or dual-species biofilms. Furthermore, a competitive interaction was observed for dual-species biofilms wherein E. coli exhibited higher proliferative capacity than S. aureus, an inverse behavior from the one observed in single-species biofilms. Therefore, either nanoHA-CHX or nanoHA-ZnO surfaces appear as promising alternatives to antibiotics for the prevention of devices-related infections avoiding the critical risk of antibiotic-resistant strains emergence. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 491-497, 2017.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/jbm.a.35925pt_PT
dc.identifier.urihttp://hdl.handle.net/10284/7836
dc.language.isoengpt_PT
dc.subjectBiofilmspt_PT
dc.subjectChlorhexidinept_PT
dc.subjectEscherichia colipt_PT
dc.subjectNanoparticlespt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectDurapatitept_PT
dc.subjectZinc Oxidept_PT
dc.titleStaphylococcus aureusandEscherichia colidual-species biofilms on nanohydroxyapatite loaded with CHX or ZnO nanoparticlespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage497pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage491pt_PT
oaire.citation.volume105pt_PT
person.familyNameGrenho
person.familyNameFerraz
person.givenNameLiliana
person.givenNameMaria Pia
person.identifierR-000-M1P
person.identifier.ciencia-idB41C-D747-BDBF
person.identifier.ciencia-id7F16-B2FB-9AD4
person.identifier.orcid0000-0001-9476-2839
person.identifier.orcid0000-0002-0274-106X
person.identifier.ridI-4540-2015
person.identifier.ridM-6316-2013
person.identifier.scopus-author-id55159751400
person.identifier.scopus-author-id7102012951
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb26a2a2c-0484-4214-97b3-b7ea69b58419
relation.isAuthorOfPublication7ba2ac59-40af-4ba1-b13e-4447f1686208
relation.isAuthorOfPublication.latestForDiscovery7ba2ac59-40af-4ba1-b13e-4447f1686208

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