Repository logo
 
Publication

Evaluation of GSH adducts of adrenaline in biological samples

2007Journal article Restricted access
Simple item page
dc.contributor.authorSilva, Renata
dc.contributor.authorBoldt, Sílvia
dc.contributor.authorCosta, Vera Marisa
dc.contributor.authorCarmo, Helena
dc.contributor.authorCarvalho, Márcia
dc.contributor.authorCarvalho, Félix
dc.contributor.authorBastos, Maria de Lourdes
dc.contributor.authorLemos-Amado, Francisco
dc.contributor.authorRemião, Fernando
dc.date.accessioned2021-07-05T09:11:21Z
dc.date.available2021-07-05T09:11:21Z
dc.date.issued2007
dc.description.abstractThe sustained high release of catecholamines to circulation is a deleterious condition that may induce toxicity, which seems to be partially related to the products formed by oxidation of catecholamines that can be further conjugated with glutathione (GSH). The aim of the present study was to develop a method for the determination of GSH adducts of adrenaline in biological samples. Two position isomers of the glutathion-S-yl-adrenaline were synthesized and characterized by HPLC using diode array, coulometric and mass detectors. A method for the extraction of these adducts from human plasma was also developed, based on adsorption to activated alumina, which showed adequate recoveries and proved to be crucial in removing interferences from plasma. The selectivity, precision and linearity of the method were all within the accepted values for these parameters. Furthermore, the sensitivity of this method allows the detection of adduct amounts that are within the range of the expected concentrations for these adducts under certain pathophysiological conditions and/or drug treatments. In conclusion, the development of this method allows the direct analysis of GSH adducts of adrenaline in human plasma, providing a valuable tool for the study of the catecholamine oxidation process and its related toxicity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/bmc.796pt_PT
dc.identifier.eissn1099-0801
dc.identifier.issn0269-3879
dc.identifier.urihttp://hdl.handle.net/10284/10050
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley & Sonspt_PT
dc.subjectGSH adductspt_PT
dc.subjectAdrenalinept_PT
dc.subjectCatecholaminespt_PT
dc.subjectOxidative stresspt_PT
dc.subjectHPLCpt_PT
dc.titleEvaluation of GSH adducts of adrenaline in biological samplespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FSAU-OBS%2F55849%2F2004/PT
oaire.citation.endPage679pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage670pt_PT
oaire.citation.titleBiomedical Chromatographypt_PT
oaire.citation.volume21pt_PT
oaire.fundingStreamPOCI
person.familyNameCarvalho
person.givenNameMarcia
person.identifier2017111
person.identifier.ciencia-id8B10-171E-E63E
person.identifier.orcid0000-0001-9884-4751
person.identifier.ridD-5999-2013
person.identifier.scopus-author-id7201413997
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3837b828-ba57-47f7-a811-cce65e4922c6
relation.isAuthorOfPublication.latestForDiscovery3837b828-ba57-47f7-a811-cce65e4922c6
relation.isProjectOfPublication4ca44686-b588-45ee-9c0d-af8cf4bdbd30
relation.isProjectOfPublication.latestForDiscovery4ca44686-b588-45ee-9c0d-af8cf4bdbd30

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Evaluation of GSH adducts of adrenaline in biological samples.pdf
Size:
427.13 KB
Format:
Adobe Portable Document Format
Download

Collections

FCS - Artigos em Revistas Científicas Internacionais com Arbitragem Científica