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Silk fibroin/nanohydroxyapatite hydrogels for promoted bioactivity and osteoblastic proliferation and differentiation of human bone marrow stromal cells

2018Journal article Open access
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dc.contributor.authorRibeiro, Marta
dc.contributor.authorFernandes, Maria H.
dc.contributor.authorBeppu, Marisa M.
dc.contributor.authorMonteiro, Fernando J.
dc.contributor.authorFerraz, Maria Pia
dc.date.accessioned2019-09-12T14:17:39Z
dc.date.available2025-01-01T01:30:18Z
dc.date.issued2018
dc.description.abstractSilk fibroin (SF) is a natural, biocompatible, and biodegradable polymer having a great potential for the successful regeneration of damaged bone tissue. In the present work, nanohydroxyapatite (nanoHA) was incorporated into SF polymer to form a bioactive composite hydrogel for applications as bone implants. The degradation and bioactive properties of SF/nanoHA composite hydrogels were evaluated. Additionally, biological investigations of human bone marrow stromal cells (hBMSCs) viability, proliferation and differentiation to the osteoblastic phenotype were conducted. The incorporation of nanoHA in SF polymer matrices improved the bioactivity of the hydrogels. The biological results highlighted that the SF/nanoHA composite hydrogels are suitable for hBMSCs attachment and proliferation, while a test for alkaline phosphatase (ALP) and bone morphogenetic protein 2 (BMP-2) expression suggested osteoblast differentiation. Additionally, a cell staining method for ALP allowed to observe cell infiltration with active production of ALP by the infiltrated cells, paving the way to use the proposed composite hydrogel for bone tissue regeneration.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.msec.2018.04.034pt_PT
dc.identifier.urihttp://hdl.handle.net/10284/7834
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectAlkaline Phosphatasept_PT
dc.subjectBiocompatible Materialspt_PT
dc.subjectBone Marrow Cellspt_PT
dc.subjectBone Morphogenetic Protein 2pt_PT
dc.subjectCell Differentiationpt_PT
dc.subjectCells, Culturedpt_PT
dc.subjectFibroinspt_PT
dc.subjectHumanspt_PT
dc.subjectHydrogelspt_PT
dc.subjectHydroxyapatitespt_PT
dc.subjectMesenchymal Stem Cellspt_PT
dc.subjectMicroscopy, Confocalpt_PT
dc.subjectMicroscopy, Electron, Scanningpt_PT
dc.subjectNanostructurespt_PT
dc.subjectOsteogenesispt_PT
dc.titleSilk fibroin/nanohydroxyapatite hydrogels for promoted bioactivity and osteoblastic proliferation and differentiation of human bone marrow stromal cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage345pt_PT
oaire.citation.startPage336pt_PT
oaire.citation.volume89pt_PT
person.familyNameFerraz
person.givenNameMaria Pia
person.identifier.ciencia-id7F16-B2FB-9AD4
person.identifier.orcid0000-0002-0274-106X
person.identifier.ridM-6316-2013
person.identifier.scopus-author-id7102012951
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication7ba2ac59-40af-4ba1-b13e-4447f1686208
relation.isAuthorOfPublication.latestForDiscovery7ba2ac59-40af-4ba1-b13e-4447f1686208

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