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Potential of FTIR spectroscopy applied to exosomes for Alzheimer’s disease discrimination: a pilot study

2020Journal article Restricted access
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dc.contributor.authorSoares Martins, Tânia
dc.contributor.authorMagalhães, Sandra
dc.contributor.authorRosa, Ilka Martins
dc.contributor.authorVogelgsang, Jonathan
dc.contributor.authorWiltfang, Jens
dc.contributor.authorDelgadillo, Ivonne
dc.contributor.authorCatita, José
dc.contributor.authorda Cruz e Silva, Odete A.B.
dc.contributor.authorNunes, Alexandra
dc.contributor.authorHenriques, Ana Gabriela
dc.date.accessioned2022-01-31T12:15:40Z
dc.date.available2022-01-31T12:15:40Z
dc.date.issued2020
dc.description.abstractAlzheimer's disease (AD) diagnosis is based on psychological and imaging tests but can also include monitoring cerebrospinal fluid (CSF) biomarkers. However, CSF based-neurochemical approaches are expensive and invasive, limiting their use to well-equipped settings. In contrast, blood-based biomarkers are minimally invasive, cost-effective, and a widely accessible alternative. Blood-derived exosomes have recently emerged as a reliable AD biomarker source, carrying disease-specific cargo. Fourier-transformed infrared (FTIR) spectroscopy meets the criteria for an ideal diagnostic methodology since it is rapid, easy to implement, and has high reproducibility. This metabolome-based technique is useful for diagnosing a broad range of diseases, although to our knowledge, no reports for FTIR spectroscopy applied to exosomes in AD exist. In this ground-breaking pilot study, FTIR spectra of serum and serum-derived exosomes from two independent cohorts were acquired and analyzed using multivariate analysis. The regional UA-cohort includes 9 individuals, clinically diagnosed with AD, mean age of 78.7 years old; and the UMG-cohort comprises 12 individuals, clinically diagnosed with AD (based on molecular and/or imaging data), mean age of 73.2 years old. Unsupervised principal component analysis of FTIR spectra of serum-derived exosomes revealed higher discriminatory value for AD cases when compared to serum as a whole. Consistently, the partial least-squares analysis revealed that serum-derived exosomes present higher correlations than serum. In addition, the second derivative peak area calculation also revealed significant differences among Controls and AD cases. The results obtained suggest that this methodology can discriminate cases from Controls and thus be potential useful to assist in AD clinical diagnosis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3233/JAD-191034pt_PT
dc.identifier.issn1387-2877
dc.identifier.pmid32039849
dc.identifier.urihttp://hdl.handle.net/10284/10680
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherIOS Presspt_PT
dc.subjectExosomespt_PT
dc.subjectBloodpt_PT
dc.subjectSerumpt_PT
dc.subjectAlzheimer’s diseasept_PT
dc.subjectBiomarkerpt_PT
dc.subjectDiagnosispt_PT
dc.titlePotential of FTIR spectroscopy applied to exosomes for Alzheimer’s disease discrimination: a pilot studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage405pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage391pt_PT
oaire.citation.titleJournal of Alzheimer's Diseasept_PT
oaire.citation.volume74pt_PT
person.familyNameMorais Catita
person.givenNameJose Antonio
person.identifierJose Catita
person.identifier.ciencia-idB41E-3287-E706
person.identifier.orcid0000-0001-7270-5702
person.identifier.scopus-author-id6507275921
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication24bb2971-32ae-429b-b397-145dacb66a5a
relation.isAuthorOfPublication.latestForDiscovery24bb2971-32ae-429b-b397-145dacb66a5a

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