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Effect of extraction methodology on the phytochemical composition for camelia sinensis “powdered tea extracts” from different provenances

dc.contributor.authorBarreira, Sérgio
dc.contributor.authorSilva, André M. N.
dc.contributor.authorMoutinho, Carla
dc.contributor.authorSeo, Ean-Jeong
dc.contributor.authorHegazy, Mohamed-Elamir F.
dc.contributor.authorEfferth, Thomas
dc.contributor.authorGomes, Lígia Rebelo
dc.date.accessioned2022-04-07T12:12:17Z
dc.date.available2022-04-07T12:12:17Z
dc.date.issued2022
dc.description.abstract(1) Background: beverages based on extracts from Camellia sinensis are popular worldwide. Due to an increasing number of processed teas on the market, there is a need to develop unified classification standards based on chemical analysis. Meanwhile, phytochemical characterizations are mainly performed on tea samples from China (~80%). Hence, data on teas of other provenances is recommended. (2) Methods: in the present investigation, we characterized lyophilised extracts obtained by infusion, maceration and methanolic extraction derived from tea samples from China, Japan, Sri Lanka and Portugal by phytochemistry (catechins, oxyaromatic acids, flavonols, alkaloids and theanine). The real benefits of drinking the tea were analysed based on the bioavailability of the determined phytochemicals. (3) Results: the infusions revealed the highest total phenolic contents (TPC) amounts, while methanolic extracts yielded the lowest. The correlation matrix indicated that the levels of phenolic compounds were similar in the infusions and methanolic samples, while extractions made by maceration were significantly different. The differences could be partially explained by the different amounts of (-)-epigallocatechin gallate (EGCG), (-)-epicatechin gallate (ECG) and gallic acids (GA). The catechin percentages were significantly lower in the macerations, especially the quantity of EGCG decreases by 4- to 5-fold after this process. (4) Conclusions: the results highlight the importance of the processing methodology to obtain “instant tea”; the composition of the extracts obtained with the same methodology is not significantly affected by the provenance of the tea. However, attention should be drawn to the specificities of the Japanese samples (the tea analysed in the present work was of Sencha quality). In contrast, the extraction methodology significantly affects the phytochemical composition, especially concerning the content of polyphenols. As such, our results indicate that instant tea classification based on chemical composition is sensible, but there is a need for a standard extraction methodology, namely concerning the temperature and time of contact of the tea leaves with the extraction solvent.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/beverages8010013pt_PT
dc.identifier.issn2306-5710
dc.identifier.urihttp://hdl.handle.net/10284/10957
dc.language.isoengpt_PT
dc.publisherMDPIpt_PT
dc.subjectPhytochemical compositionpt_PT
dc.subjectCamellia sinensispt_PT
dc.subjectHPLCpt_PT
dc.subjectCatechinspt_PT
dc.subjectNatural productpt_PT
dc.subjectTheaceaept_PT
dc.titleEffect of extraction methodology on the phytochemical composition for camelia sinensis “powdered tea extracts” from different provenancespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage13pt_PT
oaire.citation.titleBeveragespt_PT
oaire.citation.volume8pt_PT
person.familyNameBarreira
person.familyNameMoutinho
person.familyNameMaria da Silva Rebelo Gomes
person.givenNameSérgio
person.givenNameCarla
person.givenNameLígia
person.identifier.ciencia-id4514-45D3-DC11
person.identifier.ciencia-idB61F-F356-80C6
person.identifier.ciencia-idA41A-22BE-18F0
person.identifier.orcid0000-0001-5956-7501
person.identifier.orcid0000-0003-1310-4696
person.identifier.orcid0000-0002-3496-6052
person.identifier.ridM-7142-2013
person.identifier.scopus-author-id55812422900
person.identifier.scopus-author-id16064733800
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationdbe45ee6-efba-417e-92ef-53145e55db29
relation.isAuthorOfPublicationcff7638e-1f33-4384-aa73-d3acc5cca5f8
relation.isAuthorOfPublication66614656-b892-46ad-93f3-7586580fd733
relation.isAuthorOfPublication.latestForDiscoverydbe45ee6-efba-417e-92ef-53145e55db29

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