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Antibacterial activity and biocompatibility of three-dimensional nanostructured porous granules of hydroxyapatite and zinc oxide nanoparticles—anin vitroandin vivostudy

2015Journal article Restricted access
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dc.contributor.authorGrenho, Liliana
dc.contributor.authorSalgado, C. L.
dc.contributor.authorFernandes, M. H.
dc.contributor.authorMonteiro, F. J.
dc.contributor.authorFerraz, Maria Pia
dc.date.accessioned2019-09-23T07:38:35Z
dc.date.available2019-09-23T07:38:35Z
dc.date.issued2015
dc.description.abstractCeramic scaffolds are widely studied in the bone tissue engineering field due to their potential in regenerative medicine. However, adhesion of microorganisms on biomaterials with subsequent formation of antibiotic-resistant biofilms is a critical factor in implant-related infections. Therefore, new strategies are needed to address this problem. In the present study, three-dimensional and interconnected porous granules of nanostructured hydroxyapatite (nanoHA) incorporated with different amounts of zinc oxide (ZnO) nanoparticles were produced using a simple polymer sponge replication method. As in vitro experiments, granules were exposed to Staphylococcus aureus and Staphylococcus epidermidis and, after 24 h, the planktonic and sessile populations were assessed. Cytocompatibility towards osteoblast-like cells (MG63 cell line) was also evaluated for a period of 1 and 3 days, through resazurin assay and imaging flow cytometry analysis. As in vivo experiments, nanoHA porous granules with and without ZnO nanoparticles were implanted into the subcutaneous tissue in rats and their inflammatory response after 3, 7 and 30 days was examined, as well as their antibacterial activity after 1 and 3 days of S. aureus inoculation. The developed composites proved to be especially effective at reducing bacterial activity in vitro and in vivo for a weight percentage of 2% ZnO, with a low cell growth inhibition in vitro and no differences in the connective tissue growth and inflammatory response in vivo. Altogether, these results suggest that nanoHA-ZnO porous granules have a great potential to be used in orthopaedic and dental applications as a template for bone regeneration and, simultaneously, to restrain biomaterial-associated infections.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1088/0957-4484/26/31/315101pt_PT
dc.identifier.issn0957-4484
dc.identifier.urihttp://hdl.handle.net/10284/8004
dc.language.isoengpt_PT
dc.publisherIOP Publishingpt_PT
dc.relation.publisherversionhttps://iopscience.iop.org/article/10.1088/0957-4484/26/31/315101pt_PT
dc.subjectAnimalspt_PT
dc.subjectAnti-Bacterial Agentspt_PT
dc.subjectBiocompatible Materialspt_PT
dc.subjectCell Linept_PT
dc.subjectDurapatitept_PT
dc.subjectHumanspt_PT
dc.subjectIn Vitro Techniquespt_PT
dc.subjectMalept_PT
dc.subjectNanoparticlespt_PT
dc.subjectOsteoblastspt_PT
dc.subjectPorositypt_PT
dc.subjectRatspt_PT
dc.subjectRats, Wistarpt_PT
dc.subjectStaphylococcal Infectionspt_PT
dc.subjectZinc Oxidept_PT
dc.subjectNanoporespt_PT
dc.titleAntibacterial activity and biocompatibility of three-dimensional nanostructured porous granules of hydroxyapatite and zinc oxide nanoparticles—anin vitroandin vivostudypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue31pt_PT
oaire.citation.startPage315101pt_PT
oaire.citation.titleNanotechnologypt_PT
oaire.citation.volume26pt_PT
person.familyNameGrenho
person.familyNameFerraz
person.givenNameLiliana
person.givenNameMaria Pia
person.identifierR-000-M1P
person.identifier.ciencia-idB41C-D747-BDBF
person.identifier.ciencia-id7F16-B2FB-9AD4
person.identifier.orcid0000-0001-9476-2839
person.identifier.orcid0000-0002-0274-106X
person.identifier.ridI-4540-2015
person.identifier.ridM-6316-2013
person.identifier.scopus-author-id55159751400
person.identifier.scopus-author-id7102012951
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb26a2a2c-0484-4214-97b3-b7ea69b58419
relation.isAuthorOfPublication7ba2ac59-40af-4ba1-b13e-4447f1686208
relation.isAuthorOfPublication.latestForDiscovery7ba2ac59-40af-4ba1-b13e-4447f1686208

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