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A rapid and simple procedure for the establishment of human normal and cancer renal primary cell cultures from surgical specimens

2011-05Journal article Open access
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dc.contributor.authorValente, Maria João
dc.contributor.authorHenrique, Rui
dc.contributor.authorCosta, Vera L.
dc.contributor.authorJerónimo, Carmen
dc.contributor.authorCarvalho, Félix
dc.contributor.authorBastos, Maria L.
dc.contributor.authorGuedes de Pinho, Paula
dc.contributor.authorCarvalho, Márcia
dc.date.accessioned2019-10-01T16:00:31Z
dc.date.available2019-10-01T16:00:31Z
dc.date.issued2011-05
dc.description.abstractThe kidney is a target organ for the toxicity of several xenobiotics and is also highly susceptible to the development of malignant tumors. In both cases, in vitro studies provide insight to cellular damage, and represent adequate models to study either the mechanisms underlying the toxic effects of several nephrotoxicants or therapeutic approaches in renal cancer. The development of efficient methods for the establishment of human normal and tumor renal cell models is hence crucial. In this study, a technically simple and rapid protocol for the isolation and culture of human proximal tubular epithelial cells and human renal tumor cells from surgical specimens is presented. Tumor and normal tissues were processed by using the same methodology, based on mechanical disaggregation of tissue followed by enzymatic digestion and cell purification by sequential sieving. The overall procedure takes roughly one hour. The resulting cell preparations have excellent viabilities and yield. Establishment of primary cultures from all specimens was achieved successfully. The origin of primary cultured cells was established through morphological evaluation. Normal cells purity was confirmed by immunofluorescent staining and reverse transcription-polymerase chain reaction analysis for expression of specific markers.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1371/journal.pone.0019337pt_PT
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10284/8091
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPublic Library of Sciencept_PT
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0019337pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAgedpt_PT
dc.subjectCell culture techniquespt_PT
dc.subjectFemalept_PT
dc.subjectHumanspt_PT
dc.subjectKidneypt_PT
dc.subjectKidney neoplasmspt_PT
dc.subjectMalept_PT
dc.subjectMiddle agedpt_PT
dc.subjectTumor cellspt_PT
dc.subjectCulturedpt_PT
dc.titleA rapid and simple procedure for the establishment of human normal and cancer renal primary cell cultures from surgical specimenspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage8
oaire.citation.issue5pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePLOS ONEpt_PT
oaire.citation.volume6pt_PT
person.familyNameCarvalho
person.givenNameMarcia
person.identifier2017111
person.identifier.ciencia-id8B10-171E-E63E
person.identifier.orcid0000-0001-9884-4751
person.identifier.ridD-5999-2013
person.identifier.scopus-author-id7201413997
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3837b828-ba57-47f7-a811-cce65e4922c6
relation.isAuthorOfPublication.latestForDiscovery3837b828-ba57-47f7-a811-cce65e4922c6

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