Repository logo
 
Publication

Alkaloid production by a Cinchona officinalis "Ledgeriana" hairy root culture containing constitutive expression constructs of tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseus

1999Journal article Open access
Simple item page
dc.contributor.authorGeerlings, A.
dc.contributor.authorHallard, D.
dc.contributor.authorMartinez Caballero, A.
dc.contributor.authorCardoso, I. Lopes
dc.contributor.authorHeijden, R. Van Der
dc.contributor.authorVerpoorte, R.
dc.date.accessioned2020-03-05T18:32:22Z
dc.date.available2020-03-05T18:32:22Z
dc.date.issued1999
dc.date.updated2020-02-28T16:58:51Z
dc.description.abstractCinchona officinalis ‘Ledgeriana’, former called Cinchona ledgeriana, hairy roots were initiated containing constitutive-expression constructs of cDNAs encoding the enzymes tryptophan decarboxylase (TDC) and strictosidine synthase (STR) from Catharanthus roseus, two key enzymes in terpenoid indole and quinoline alkaloid biosynthesis. The successful integration of these genes and the reporter gene gus-int was demonstrated using Southern blotting and the polymerase chain reaction. The products of TDC and STR, tryptamine and strictosidine, were found in high amounts, 1200 and 1950 mg g–1 dry weight, respectively. Quinine and quinidine levels were found to rise up to 500 and 1000 mg g–1 dry weight, respectively. The results show that genetic engineering with multiple genes is well possible in hairy roots of C. officinalis. However, 1 year after analyzing the hairy roots for the first time, they had completely lost their capacity to accumulate alkaloids.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGeerlings, A., Hallard, D., Martinez Caballero, A., Lopes Cardoso, I., van der Heijden, R., & Verpoorte, R. (1999). Alkaloid production by a Cinchona officinalis 'Ledgeriana' hairy root culture containing constitutive expression constructs of tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseus. Plant Cell Reports, 19(2), 191–196. https://doi.org/10.1007/s002990050732
dc.identifier.slugcv-prod-368668
dc.identifier.urihttp://hdl.handle.net/10284/8622
dc.language.isoengpt_PT
dc.subjectQuinoline alkaloidspt_PT
dc.subjectGenetic engineeringpt_PT
dc.subjectCatharanthus roseuspt_PT
dc.subjectCinchona officinalis "Ledgeriana"pt_PT
dc.subjectTerpenoid indole alkaloidspt_PT
dc.titleAlkaloid production by a Cinchona officinalis "Ledgeriana" hairy root culture containing constitutive expression constructs of tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage196pt_PT
oaire.citation.startPage191pt_PT
oaire.citation.titlePlant Cell Reportspt_PT
oaire.citation.volume19pt_PT
person.familyNameLopes Cardoso
person.givenNameInês
person.identifier.ciencia-idF21F-16B4-3715
person.identifier.orcid0000-0002-0693-9831
person.identifier.ridM-7156-2013
person.identifier.scopus-author-id54973754300
rcaap.cv.cienciaidF21F-16B4-3715 | Maria Inês de Avelar Lopes Cardoso
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf39fa18f-2d23-4690-bfa2-f8ba53e40775
relation.isAuthorOfPublication.latestForDiscoveryf39fa18f-2d23-4690-bfa2-f8ba53e40775

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
GeerlingsLopes_Cardoso99.pdf
Size:
268.58 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.64 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

FCS (DCM)- Artigos em Revistas Científicas Internacionais com Arbitragem Científica