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Alkaloid production by a Cinchona officinalis "Ledgeriana" hairy root culture containing constitutive expression constructs of tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseus
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Orientador(es)
Resumo(s)
Cinchona officinalis ‘Ledgeriana’, former
called Cinchona ledgeriana, hairy roots were initiated
containing constitutive-expression constructs of cDNAs
encoding the enzymes tryptophan decarboxylase
(TDC) and strictosidine synthase (STR) from Catharanthus roseus, two key enzymes in terpenoid indole
and quinoline alkaloid biosynthesis. The successful
integration of these genes and the reporter gene gus-int
was demonstrated using Southern blotting and the
polymerase chain reaction. The products of TDC and
STR, tryptamine and strictosidine, were found in high
amounts, 1200 and 1950 mg g–1 dry weight, respectively.
Quinine and quinidine levels were found to rise up to
500 and 1000 mg g–1 dry weight, respectively. The
results show that genetic engineering with multiple
genes is well possible in hairy roots of C. officinalis.
However, 1 year after analyzing the hairy roots for the
first time, they had completely lost their capacity to
accumulate alkaloids.
Descrição
Palavras-chave
Quinoline alkaloids Genetic engineering Catharanthus roseus Cinchona officinalis "Ledgeriana" Terpenoid indole alkaloids
Contexto Educativo
Citação
Geerlings, A., Hallard, D., Martinez Caballero, A., Lopes Cardoso, I., van der Heijden, R., & Verpoorte, R. (1999). Alkaloid production by a Cinchona officinalis 'Ledgeriana' hairy root culture containing constitutive expression constructs of tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseus. Plant Cell Reports, 19(2), 191–196. https://doi.org/10.1007/s002990050732