Repository logo
 
Publication

Risperidone release from solid lipid nanoparticles (SLN): validated HPLC method and modelling kinetic profile

2012-10-01Journal article Restricted access
Simple item page
dc.contributor.authorSilva, Ana Catarina
dc.contributor.authorLopes, Carla Martins
dc.contributor.authorFonseca, J.
dc.contributor.authorSoares, M.E.
dc.contributor.authorSantos, D.
dc.contributor.authorSouto, Eliana B.
dc.contributor.authorFerreira, D.
dc.date.accessioned2020-10-14T09:22:32Z
dc.date.available2020-10-14T09:22:32Z
dc.date.issued2012-10-01
dc.date.updated2020-07-24T16:22:09Z
dc.description.abstractA simple reverse-phase (RP) high performance liquid chromatography (HPLC) method was developed and validated, according to the International Harmonisation Guidelines (ICH), for the determination of risperidone (RISP) from solid lipid nanoparticles (SLN). Chromatographic runs were performed on a RP-C18 column, using an isocratic mobile phase of methanol, acetate buffer (0.05 M; pH 4.6) and triethylamine (60:40:0.02, v/v/v). The flow rate was 1 ml/min, the run time was 10 min and the RISP absorbance was measured at 280 nm, using UV detection. A linear response was obtained for a RISP concentration range of 0.25 - 10.00 g/ml (R2 = 0.9996), with a detection and quantification limits of 0.011 and 0.034 g/ml, respectively. The method was shown to be specific, precise at the intra-day (RSD < 0.796%) and inter-day (RSD < 0.331%) levels, and accurate with recoveries between 86.86 - 100.3% (RSD < 0.613%). Method robustness was observed as well. The suitability of the method for RISP quantifications was assessed by the determination of encapsulation parameters (encapsulation efficiency and drug loading) and by studying the RISP release profile from SLN. Kinetic models (zero order, Higuchi, Korsmeyer-Peppas and Baker-Lonsdale) were used to fit the obtained release profile and to predict the in vivo performance of RISP-loaded SLN. A combined pattern of diffusion and erosion release mechanism (anomalous non-Fickian transport) was found for the RISP-loaded SLN, which shows the ability of the system for controlled drug release.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.2174/157341212803341663pt_PT
dc.identifier.eid2-s2.0-84857786253
dc.identifier.issn1573-4129
dc.identifier.issn1875-676X
dc.identifier.slugcv-prod-1252837
dc.identifier.urihttp://hdl.handle.net/10284/9055
dc.language.isoengpt_PT
dc.publisherBentham Science Publisherspt_PT
dc.subjectControlled drug releasept_PT
dc.subjectEncapsulation efficiencypt_PT
dc.subjectEncapsulation parameterspt_PT
dc.subjectHigh performance liquid chromatographypt_PT
dc.subjectLoading capacitypt_PT
dc.subjectKinetic modelpt_PT
dc.subjectOral drug deliverypt_PT
dc.subjectSolid lipid nanoparticlespt_PT
dc.subjectRelease profilept_PT
dc.subjectRisperidonept_PT
dc.titleRisperidone release from solid lipid nanoparticles (SLN): validated HPLC method and modelling kinetic profilept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage316pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage307pt_PT
oaire.citation.titleCurrent Pharmaceutical Analysispt_PT
oaire.citation.volume8pt_PT
person.familyNameSilva
person.familyNameLopes
person.familyNameSouto
person.givenNameAna Catarina
person.givenNameCarla
person.givenNameEliana B.
person.identifier953153
person.identifier.ciencia-id5C1D-ED22-0D64
person.identifier.ciencia-id901D-160C-633E
person.identifier.ciencia-id8D1B-21CC-90F7
person.identifier.orcid0000-0001-6923-0232
person.identifier.orcid0000-0001-5080-032X
person.identifier.orcid0000-0002-9737-6017
person.identifier.ridF-1875-2017
person.identifier.ridM-4689-2016
person.identifier.ridL-9813-2014
person.identifier.scopus-author-id57028697500
person.identifier.scopus-author-id26649517700
person.identifier.scopus-author-id8839435500
rcaap.cv.cienciaid901D-160C-633E | Carla Martins Lopes
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication67e7f707-32ec-444a-a20d-a17f8e6e7c21
relation.isAuthorOfPublicationd2a48cfe-9258-4916-99be-bbcb710f6605
relation.isAuthorOfPublicationb9f031fa-7f68-4196-b9c6-9f17cd542f12
relation.isAuthorOfPublication.latestForDiscoveryb9f031fa-7f68-4196-b9c6-9f17cd542f12

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Risperidone release from solid lipid nanoparticles (SLN)_2012.pdf
Size:
189.88 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.64 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

FCS (DCF) - Artigos em Revistas Científicas Internacionais com Arbitragem Científica