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Effects of cerium dioxide nanoparticles in Oncorhynchus mykiss liver and kidney after an acute exposure: assessment of oxidative stress, genotoxicity and histological alterations
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Atualmente, as nanopartículas de dióxido de cério (CeO2-NPs) apresentam inúmeras aplicações que vão desde a indústria até ao uso doméstico conduzindo à presença ubíqua deste composto no ambiente, em particular no compartimento aquático. Perante os poucos estudos existentes sobre o impacto destas nanopartículas no meio aquático, este trabalho teve como objetivo estudar a toxicidade de CeO2-NPs no fígado e rim de Oncorhynchus mykiss (truta arco-íris) após exposição aguda (96h) a três diferentes concentrações (0,25; 2,50 e 25,00 mg/L) deste composto. Avaliou-se a genotoxicidade (comet assay), a resposta ao stress oxidativo (Catalase CAT, Glutationa-S-transferases GSTs), a peroxidação lipídica (substâncias reativas ao ácido tiobarbitúrico TBARS), os danos histopatológicos e o efeito na atividade da Na+/K+-ATPase no rim. A exposição ao CeO2-NPs resultou em genotoxicidade nos hepatócitos em todos os grupos expostos ao composto, na inibição da CAT na concentração mais alta (25,00 mg/L) e em alterações histológicas em todos os grupos expostos, com predominância de alterações progressivas, circulatórias e inflamatórias. Porém TBARS, GSTs e Na+/K+-ATPase não apresentaram diferenças significativas comparativamente ao grupo controlo (indivíduos não expostos). Os resultados obtidos neste estudo sugerem assim que CeO2-NPs é capaz de causar genotoxicidade, desequilíbrio da CAT e alterações histológicas em fígado de truta arco-íris.
At present cerium oxide nanoparticles (CeO2-NPs) have numerous applications ranging from industry to the household, leading to its wide presence in the environment, namely in the aquatic compartment. The hereby study aimed to assess the toxic effects of CeO2-NPs in Oncorhynchus mykiss liver and kidney after an acute exposure (96h) to three different concentrations (0.25, 2.50 and 25.00 mg/L) of the compound. Genotoxicity (comet assay), oxidative stress response (Catalase CAT; Glutathione S-Transferases GSTs), lipidic peroxidation (Thiobarbituric Acid Reactive Substances TBARS), histopathology and Na+/K+-ATPase activity were evaluated. CeO2-NPs exposure resulted in genotoxic damage in all exposure treatments, inhibition of CAT in the highest concentration (25.00 mg/L) and histopathological changes in all exposure concentrations with predominance of progressive, circulatory and inflammatory alterations. However TBARS, GSTs and Na+/K+-ATPase activity showed no significant differences comparatively to the control (unexposed) group. The results suggest that CeO2-NPs are able to cause genotoxicity, CAT impairment and histological alterations in the liver of rainbow trout.
At present cerium oxide nanoparticles (CeO2-NPs) have numerous applications ranging from industry to the household, leading to its wide presence in the environment, namely in the aquatic compartment. The hereby study aimed to assess the toxic effects of CeO2-NPs in Oncorhynchus mykiss liver and kidney after an acute exposure (96h) to three different concentrations (0.25, 2.50 and 25.00 mg/L) of the compound. Genotoxicity (comet assay), oxidative stress response (Catalase CAT; Glutathione S-Transferases GSTs), lipidic peroxidation (Thiobarbituric Acid Reactive Substances TBARS), histopathology and Na+/K+-ATPase activity were evaluated. CeO2-NPs exposure resulted in genotoxic damage in all exposure treatments, inhibition of CAT in the highest concentration (25.00 mg/L) and histopathological changes in all exposure concentrations with predominance of progressive, circulatory and inflammatory alterations. However TBARS, GSTs and Na+/K+-ATPase activity showed no significant differences comparatively to the control (unexposed) group. The results suggest that CeO2-NPs are able to cause genotoxicity, CAT impairment and histological alterations in the liver of rainbow trout.
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Peixes Exposição Nanopartículas Biomarcadores Fish Exposure Nanoparticles Biomarkers