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Oxidation process of adrenaline in freshly isolated rat cardiomyocytes: formation of adrenochrome, quinoproteins, and GSH adduct

2007Journal article Restricted access
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dc.contributor.authorCosta, Vera Marisa
dc.contributor.authorSilva, Renata
dc.contributor.authorFerreira, Luísa Maria
dc.contributor.authorBranco, Paula Sério
dc.contributor.authorCarvalho, Félix
dc.contributor.authorBastos, Maria de Lourdes
dc.contributor.authorCarvalho, Rui Albuquerque
dc.contributor.authorCarvalho, Márcia
dc.contributor.authorRemião, Fernando
dc.date.accessioned2021-07-01T16:46:11Z
dc.date.available2021-07-01T16:46:11Z
dc.date.issued2007
dc.description.abstractHigh concentrations of circulating biogenic catecholamines often exist during the course of several cardiovascular disorders. Additionally, coronary dysfunctions are prominent and frequently related to the ischemic and reperfusion phenomenon (I/R) in the heart, which leads to the release of large amounts of catecholamines, namely adrenaline, and to a sustained generation of reactive oxygen species (ROS). Thus, this work aimed to study the toxicity of adrenaline either alone or in the presence of a system capable of generating ROS [xanthine with xanthine oxidase (X/XO)], in freshly isolated, calcium tolerant cardiomyocytes from adult rats. Studies were performed for 3 h, and cardiomyocyte viability, ATP level, lipid peroxidation, protein carbonylation content, and glutathione status were evaluated, in addition to the formation of adrenaline's oxidation products and quinoproteins. Intracellular GSH levels were time-dependently depleted with no GSSG formation when cardiomyocytes were exposed to adrenaline or to adrenaline with X/XO. Meanwhile, a time-dependent increase in the rate of formation of adrenochrome and quinoproteins was observed. Additionally, as a new outcome, 5-(glutathion- S-yl)adrenaline, an adrenaline adduct of glutathione, was identified and quantified. Noteworthy is the fact that the exposure to adrenaline alone promotes a higher rate of formation of quinoproteins and glutathione adduct, while adrenochrome formation is favored where ROS production is stimulated. This study shows that the redox status of the surrounding environment greatly influences adrenaline's oxidation pathway, which may trigger cellular changes responsible for cardiotoxicity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1021/tx7000916pt_PT
dc.identifier.eissn1520-5010
dc.identifier.issn0893-228X
dc.identifier.urihttp://hdl.handle.net/10284/10007
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.relationThis work received financial support from “Fundação para a Ciência e Tecnologia” (FCT) and “Programa Operacional Ciência e Inovação 2010”, Portugal (POCI-2010), through FEDER European Community cofunding (Project POCI/SAU-OBS/55849/2004). V.M.C. acknowledges FCT for her Ph.D. grant (SFRD/BD/17677/ 2004).pt_PT
dc.subjectAdenosine triphosphatept_PT
dc.subjectAdrenergic agonistspt_PT
dc.subjectAdrenochromept_PT
dc.subjectAlcohol oxidoreductasespt_PT
dc.subjectAnimalspt_PT
dc.subjectCalciumpt_PT
dc.subjectCell survivalpt_PT
dc.subjectDose-response relationshippt_PT
dc.subjectEpinephrinept_PT
dc.subjectGlutathionept_PT
dc.subjectIntracellular membranespt_PT
dc.subjectKineticspt_PT
dc.subjectMyocytespt_PT
dc.subjectOxidation-reductionpt_PT
dc.subjectRatspt_PT
dc.subjectSprague-Dawleypt_PT
dc.subjectReactive oxygen speciespt_PT
dc.subjectXanthinept_PT
dc.subjectXanthine oxidasept_PT
dc.subjectCardiacpt_PT
dc.subjectDrugspt_PT
dc.titleOxidation process of adrenaline in freshly isolated rat cardiomyocytes: formation of adrenochrome, quinoproteins, and GSH adductpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1191pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage1183pt_PT
oaire.citation.titleChemical Research in Toxicologypt_PT
oaire.citation.volume20pt_PT
person.familyNameCarvalho
person.givenNameMarcia
person.identifier2017111
person.identifier.ciencia-id8B10-171E-E63E
person.identifier.orcid0000-0001-9884-4751
person.identifier.ridD-5999-2013
person.identifier.scopus-author-id7201413997
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3837b828-ba57-47f7-a811-cce65e4922c6
relation.isAuthorOfPublication.latestForDiscovery3837b828-ba57-47f7-a811-cce65e4922c6

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