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Human cancer cell antiproliferative and antioxidant activities of Juglans regia L.

2010Journal article Restricted access
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dc.contributor.authorCarvalho, Márcia
dc.contributor.authorFerreira, Pedro J.
dc.contributor.authorMendes, Vanda S.
dc.contributor.authorSilva, Renata
dc.contributor.authorPereira, José A.
dc.contributor.authorJerónimo, Carmen
dc.contributor.authorSilva, Branca M.
dc.date.accessioned2021-07-02T13:57:39Z
dc.date.available2021-07-02T13:57:39Z
dc.date.issued2010
dc.description.abstractSeveral studies suggest that regular consumption of nuts, mostly walnuts, may have beneficial effects against oxidative stress mediated diseases such as cardiovascular disease and cancer. Walnuts contain several phenolic compounds which are thought to contribute to their biological properties. The present study reports the total phenolic contents and antioxidant properties of methanolic and petroleum ether extracts obtained from walnut (Juglans regia L.) seed, green husk and leaf. The total phenolic contents were determined by the Folin-Ciocalteu method and the antioxidant activities assessed by the ability to quench the stable free radical 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis of human erythrocytes. Methanolic seed extract presented the highest total phenolic content (116 mg GAE/g of extract) and DPPH scavenging activity (EC(50) of 0.143 mg/mL), followed by leaf and green husk. In petroleum ether extracts, antioxidant action was much lower or absent. Under the oxidative action of AAPH, all methanolic extracts significantly protected the erythrocyte membrane from hemolysis in a time- and concentration-dependent manner, although leaf extract inhibitory efficiency was much stronger (IC(50) of 0.060 mg/mL) than that observed for green husks and seeds (IC(50) of 0.127 and 0.121 mg/mL, respectively). Walnut methanolic extracts were also assayed for their antiproliferative effectiveness using human renal cancer cell lines A-498 and 769-P and the colon cancer cell line Caco-2. All extracts showed concentration-dependent growth inhibition toward human kidney and colon cancer cells. Concerning A-498 renal cancer cells, all extracts exhibited similar growth inhibition activity (IC(50) values between 0.226 and 0.291 mg/mL), while for both 769-P renal and Caco-2 colon cancer cells, walnut leaf extract showed a higher antiproliferative efficiency (IC(50) values of 0.352 and 0.229 mg/mL, respectively) than green husk or seed extracts. The results obtained herein strongly indicate that walnut tree constitute an excellent source of effective natural antioxidants and chemopreventive agents.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.fct.2009.10.043pt_PT
dc.identifier.issn0278-6915
dc.identifier.urihttp://hdl.handle.net/10284/10030
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectJuglans regia L.pt_PT
dc.subjectLeafpt_PT
dc.subjectGreen huskpt_PT
dc.subjectWalnutpt_PT
dc.subjectAntiproliferative activitypt_PT
dc.subjectAntioxidantpt_PT
dc.titleHuman cancer cell antiproliferative and antioxidant activities of Juglans regia L.pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage447pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage441pt_PT
oaire.citation.titleFood and Chemical Toxicologypt_PT
oaire.citation.volume48pt_PT
person.familyNameCarvalho
person.familyNameSilva
person.givenNameMarcia
person.givenNameBranca Maria
person.identifier2017111
person.identifier1033309
person.identifier.ciencia-id8B10-171E-E63E
person.identifier.ciencia-idBD1F-B24D-89A9
person.identifier.orcid0000-0001-9884-4751
person.identifier.orcid0000-0002-5610-8024
person.identifier.ridD-5999-2013
person.identifier.scopus-author-id7201413997
person.identifier.scopus-author-id7005615589
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3837b828-ba57-47f7-a811-cce65e4922c6
relation.isAuthorOfPublication26c68872-1849-4895-a48d-72ccffbc02df
relation.isAuthorOfPublication.latestForDiscovery3837b828-ba57-47f7-a811-cce65e4922c6

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