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Publication
Mecanismos de degradação das esponjas hemostáticas em cirurgia oral: revisão sistemática
| Name: | Description: | Size: | Format: | |
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| 38157 | 413.44 KB | Adobe PDF |
Advisor(s)
Abstract(s)
Objetivo: Identificar os métodos utilizados nos estudos da degradação dos materiais de colagénio ou gelatina e as possíveis falhas associadas.
Metodologia: Pesquisa bibliográfica de artigos publicados nas bases de dados eletrónicas PubMed, B-On e Cochrane Library. A identificação e exclusão da evidência seguiu os critérios PRISMA, tendo sido estipulados fatores de inclusão e de exclusão específicos. A avaliação metodológica dos estudos foi realizada através da Escala de QUIN.
Resultados: Dos 11926 estudos identificados, 9 foram incluídos. Identificaram-se como limitações as concentrações e tipos de colagenase utilizados e os períodos de avaliação da degradação escolhidos. Evidenciou-se ainda a falta de uniformidade nos protocolos empregados, que variaram entre cada estudo.
Conclusão: Futuramente, os estudos in-vitro da biodegradação enzimática dos biomateriais de colagénio e gelatina devem usar uma concentração de 0,02 U de colagenase (MMP-8)/mL de saliva humana a 37 ºC e realizar momentos de avaliação a cada 24 h, de forma a produzir resultados mais fidedignos que possam ser traduzidos para conclusões clínicas.
Objective: To identify the methods used in the studies of the degradation of collagen or gelatin materials and the possible associated flaws. Methodology: Bibliographic search of articles published in the electronic databases PubMed, B-On and Cochrane Library. The identification and exclusion of evidence followed the PRISMA criteria, with the stipulation of specific inclusion and exclusion factors. The methodological evaluation of the studies was performed using the QUIN Scale. Results: Of the 11926 identified studies, 9 were included. The concentrations and types of collagenase used and the chosen degradation evaluation periods were identified as limitations. The lack of uniformity in the protocols used, which varied between each study, was also evident. Conclusion: In the future, in-vitro studies of the enzymatic biodegradation of collagen and gelatin biomaterials should use a concentration of 0.02 U of collagenase (MMP-8)/mL of human saliva at 37 °C and perform evaluation moments every 24 h, so to produce more reliable results that can be translated into clinical conclusions.
Objective: To identify the methods used in the studies of the degradation of collagen or gelatin materials and the possible associated flaws. Methodology: Bibliographic search of articles published in the electronic databases PubMed, B-On and Cochrane Library. The identification and exclusion of evidence followed the PRISMA criteria, with the stipulation of specific inclusion and exclusion factors. The methodological evaluation of the studies was performed using the QUIN Scale. Results: Of the 11926 identified studies, 9 were included. The concentrations and types of collagenase used and the chosen degradation evaluation periods were identified as limitations. The lack of uniformity in the protocols used, which varied between each study, was also evident. Conclusion: In the future, in-vitro studies of the enzymatic biodegradation of collagen and gelatin biomaterials should use a concentration of 0.02 U of collagenase (MMP-8)/mL of human saliva at 37 °C and perform evaluation moments every 24 h, so to produce more reliable results that can be translated into clinical conclusions.
Description
Keywords
Biodegradação Colagenase Degradação enzimática Materiais de colagénio Materiais de gelatina Testes de biodegradação in-vitro Biodegradation Collagenase Collagen materials Enzymatic degradation Gelatin materials In-vitro biodegradation assays