Loading...
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 1.51 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Bacterial infections and malaria remain a major public health problem due to the emergence and spread of drug resistant strains. There is an urgent need to investigate new sources of antibacterial and antimalarial drugs, which are more effective. One of the potential sources of antibacterial and antimalarial drugs is traditional medicinal plants. Our ethnopharmacological studies, in several Angolan regions, showed that Cymbopogon citratus (DC) Stapf., has high bioactivity against bacterial infections and malaria. The constituents of Cymbopogon citratus essential oil (CCEO), obtained by hydro-distillation, were analysed by GC and GC-MS and identified from their retention indices and mass spectra. The antimicrobial activity of CCEO and major natural volatile compounds were tested against several bacterial strains. The antimalarial assays where performed in continuous in vitro cultures of asexual erythrocyte stages of P. falciparum using the parasite lactate dehydrogenase assay. CCEO exhibits broad-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria. Our results showed that multi-resistant Stafilococus aureus (MRSA) isolates were more sensitive to CCEO than non-MRSA. When tested against MRSA resistant to amoxicillin-clavulanic acid combination, penicillin G and methicillin, CCEO shows a significant increase in bactericidal activity when compared with the commercial antibiotics. The same results were obtained using vancomycin resistant S. epidermidis and other strains. Regarding the anti-malaria activity, test samples where considered active for in vitro antimalarial activity exhibiting IC50 values of 5.34±1.01 μg/ml and 7.06±0.47 μg/ml. Our work shows that CCEO has higher antibacterial activity than commercial antibiotics against MRSA strains, as well as antimalarial activity. This offers opportunities for clinical treatments since preliminary in vivo studies showed no toxic effects and high antimalarial activity.
Description
Keywords
Cymbopogon citratus Essential oil Antibacterial activity Multi-resistant bacteria P. falciparum Malaria
Citation
Pires, P.C., Sekhoacha, M., Matsabisa, M., Tselanyane, M., Vinha, A.F., Soares, M.O. 2017. Antibacterial and antimalarial activity of Angolan Cymbopogon citratus essential oil, In: Antimicrobial Research: Novel Bioknowledge and Educational Programs. Formatex Research Center, pp. 62-67. ISBN: 978-84-947512-0-2
Publisher
Formatex Research Center