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Analysis of volatile human urinary metabolome by solid-phase microextraction in combination with gas chromatography–mass spectrometry for biomarker discovery: application in a pilot study to discriminate patients with renal cell carcinoma

2014Journal article Restricted access
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dc.contributor.authorMonteiro, Márcia
dc.contributor.authorCarvalho, Márcia
dc.contributor.authorHenrique, Rui
dc.contributor.authorJerónimo, Carmen
dc.contributor.authorMoreira, Nathalie
dc.contributor.authorBastos, Maria de Lourdes
dc.contributor.authorGuedes de Pinho, Paula
dc.date.accessioned2021-07-02T11:23:06Z
dc.date.available2021-07-02T11:23:06Z
dc.date.issued2014
dc.description.abstractA new and simple analytical approach consisting of headspace-solid phase microextraction (HS-SPME) sampling coupled with gas chromatography-ion trap/mass spectrometry (GC-IT/MS) was developed to study the volatile human urinary metabolome. A central composite design (CCD) was used in the optimisation of extraction conditions. Fibre selection and evaluation of pH influence were performed using an univariate mode and the influence of other parameters, such as the time and temperature of extraction, time of incubation and salt addition, that affect the efficiency of the SPME sampling, was carried out using a CCD. With a sample volume of 2 mL, the optimal conditions in terms of total response values and reproducibility were achieved by performing analyses with a divinylbenzene/polydimethylsiloxane (DVB/PDMS) fibre, in an acidic pH (pH 2) with the addition of 0.59 g of NaCl, allowing the sample to equilibrate for 9 min and extracting at 68 °C for 24 min. The applicability of the optimised method was then tested in a pilot non-target analysis of urine samples obtained from patients with renal cell carcinoma (RCC) and healthy individuals. Chemometric unsupervised analyses performed on the volatile pattern acquired for these samples clearly showed the potential of volatile urinary metabolome to discriminate between RCC and control patients.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ejca.2014.04.011pt_PT
dc.identifier.issn0959-8049
dc.identifier.urihttp://hdl.handle.net/10284/10020
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation2013 - Strategic Project
dc.subjectUrinept_PT
dc.subjectVolatile metabolomic profilept_PT
dc.subjectRenal cell carcinomapt_PT
dc.subjectCentral composite designpt_PT
dc.subjectHeadspace solid-phase microextractionpt_PT
dc.subjectGas chromatography-ion trap/mass spectrometrypt_PT
dc.titleAnalysis of volatile human urinary metabolome by solid-phase microextraction in combination with gas chromatography–mass spectrometry for biomarker discovery: application in a pilot study to discriminate patients with renal cell carcinomapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitle2013 - Strategic Project
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FEQB%2FLA0006%2F2013/PT
oaire.citation.endPage2002pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage1993pt_PT
oaire.citation.titleEuropean Journal of Cancerpt_PT
oaire.citation.volume50pt_PT
oaire.fundingStream6820 - DCRRNI ID
person.familyNameCarvalho
person.givenNameMarcia
person.identifier2017111
person.identifier.ciencia-id8B10-171E-E63E
person.identifier.orcid0000-0001-9884-4751
person.identifier.ridD-5999-2013
person.identifier.scopus-author-id7201413997
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3837b828-ba57-47f7-a811-cce65e4922c6
relation.isAuthorOfPublication.latestForDiscovery3837b828-ba57-47f7-a811-cce65e4922c6
relation.isProjectOfPublicationd95a62fe-3df1-48d6-b300-1ec7283d043b
relation.isProjectOfPublication.latestForDiscoveryd95a62fe-3df1-48d6-b300-1ec7283d043b

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