FCS (DCM)- Artigos em Revistas Científicas Internacionais com Arbitragem Científica
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- Amelogenin-based molecular methods for sexual dimorphism identification: protocol of a scoping reviewPublication . Lopes Cardoso, Inês; Moreira, Teresa; Dupuis, Clarisse; Correia de Castro, Filipe; Guimarães, Maria InêsForensic dentistry and sexual dimorphism are distinct concepts. Still, they are related due to the usefulness that the first may have in the second, and this review focuses on them. A scoping review will be performed according to the Joanna Briggs Institute’s methodology. Scientific databases and grey literature will be used, and the following keywords will be applied: amelogenin, analyses, sex determination, and human identification. This scoping review will include in vitro studies concerning the goal of this review. This scoping review will deepen our knowledge concerning using teeth and amelogenin genes in sex identification in a forensic context. According to the available data, it will help implement guidelines for human remains identification. This protocol was registered with the Open Science Framework.
- Comparison of spectroscopic and molecular methods for estimating age and sex for medico-legal purposes: protocol for a systematic reviewPublication . Lopes Cardoso, Inês; Loibl, Beatriz; Rodrigues, Carolina; Moreira, Teresa; Guimarães, Maria InêsIntroduction: In forensic contexts, accurate estimation of age and sex is fundamental for the identification of human remains. Spectroscopic methods, offering non-destructive and precise data insights, have emerged as promising alternatives/complements to traditional morphological and genetic techniques. Objective: This protocol outlines a systematic review aimed at evaluating the effectiveness of spectroscopic and molecular methods for estimating age and/or sex based on dental samples in medicolegal settings. Methodology: This review will be conducted in accordance with the PRISMA 2020 guidelines. Databases to be searched include PubMed, Web of Science, ScienceDirect, LILACS (via BVS), Cochrane Library (Protocols), MEDLINE (via EBSCOhost), Academic Search Complete (via EBSCOhost), CINAHL (via EBSCOhost) and grey literature. The eligibility criteria follow the PICOS framework. Risk of bias will be assessed using the QUIN tool for in vitro studies. Data synthesis will involve both quantitative and qualitative approaches. The protocol of this review has been registered in PROSPERO (CRD420250650858). Results: This review is expected to identify a range of spectroscopic techniques applied to dental samples for age and sex estimation, including mass spectrometry, Raman spectroscopy, FTIR spectroscopy, and X-ray fluorescence, as well as molecular methods such as amelogenin analysis, DNA methylation, and telomere length assessment. The evidence is anticipated to vary regarding study design, sample characteristics, analytical protocols, and reported outcome measures. The review will synthesize the available evidence on the accuracy, applicability, and methodological characteristics of these approaches within medico-legal contexts. Conclusion: This systematic review will provide a comprehensive overview of the available evidence regarding the use of spectroscopic and molecular methods for age and sex estimation from dental samples. By identifying the methodologies currently employed, their reported outcomes, and existing research gaps, the review may contribute to future research directions and support evidence-based decision-making in forensic and medico-legal practice, particularly in contexts where non-destructive analytical approaches are desirable.
- Degree of contamination of Gutta-Percha points by Staphylococcus aureus (MRSA/MSSA) strainsPublication . Moura Teles, Ana; Pina, Cristina; Lopes Cardoso, Inês; Tramontana, Antea; Cardoso, Miguel; Duarte, Ana Sofia; Bartolomeu, Maria; Noites, RitaMethicillin-resistant Staphylococcus aureus (MRSA) is considered one of the most harmful bacteria to human health. Dentistry, like all healthcare disciplines, places great emphasis on preventing scenarios that may result in cross-infection. Although various tested and already used materials are suitable for filling the root canal system, Gutta-Percha (GP) remains the preferred and widely accepted gold standard. Objective: We performed an in vitro analysis of the contamination of GP points, regarding the strains of Methicillin-resistant (MRSA) and Methicillin-sensitive (MSSA) Staphylococcus aureus, using classical microbiology methods and molecular biology techniques. Methods: Gutta-Percha points of two different brands from opened packages (already in use for 1 month) were collected for analysis. The assessment involved incubating the GP points in Brain Heart Infusion (BHI) medium to detect microbial growth. Growing microorganisms were plated on a selective and differential chromogenic medium for MRSA/MSSA strains, and the identification of isolates was confirmed by Polymerase Chain Reaction (PCR). In the case of microbial growth, the GP point was submitted to a disinfection protocol. Results: From the 315 collected GP points, only 6 (1.9%) resulted in being positive for microbial growth. After confirmation by PCR, only one sample of the six GP points was contaminated by MRSA, and the remaining five were MSSA-contaminated. The disinfection protocol was effective in all contaminated GP points. Conclusions: The Gutta-Percha points from opened pre-sterilized packages showed a very low degree of contamination by MRSA/MSSA. However, the detection of MSSA and MRSA strains raises concerns about potential contamination in dental clinic environments, and this risk cannot be considered negligible.
- Determination of sexual dimorphism through molecular methods: a scoping reviewPublication . Guimarães, Maria Inês; Moreira, Teresa; Dupuis, Clarisse; Lopes Cardoso, InêsBackground: Sexual dimorphism is crucial in forensic investigations. Molecular methods involving amelogenin, a protein in tooth enamel, are used to determine sexual dimorphism by extracting DNA from teeth, amplifying the gene coding for amelogenin through PCR, and analyzing PCR product sizes to identify X and/or Y chromosomes. Objective: This scoping review explored scientific studies using the amelogenin gene to determine sex in forensic dentistry. It addressed the research question: do molecular methods determine sexual dimorphism for forensic identification? Material and methods: A literature review (1996–2024) was conducted using PubMed, MEDLINE (via BVS), and CINAHL (via EBSCO host). Inclusion and exclusion criteria guided the selection process, summarized in a PRISMA flowchart. A PCC (Population-Concept-Context) strategy was applied to formulate the research question. Results: Thirteen of the 1091 articles initially considered met the inclusion and exclusion criteria. These studies examined forensic dentistry and sex determination through amelogenin identification. They were categorized into “with treatment” (7 studies) and “without treatment” (6 studies), and all involved in vitro research. Conclusion: Molecular methods targeting the amelogenin gene on the X and Y chromosomes offer an accurate and reliable approach to determining sex.
- Genetic involvement in dental treatment fearPublication . Khodabakhshi, Donja; Pina, Cristina; Moura Teles, Ana; Lopes Cardoso, InêsBeing a global health concern, dental fear and anxiety is estimated to affect at least one in ten individuals all around the globe. Ensuing the vicious circle of dental fear comes detrimental outcomes in quality of life. Despite the wide range of knowledge concerning the environmental factors that contribute to the acquisition of dental fear and anxiety, the prevalence has not decreased, giving researchers the possibility to highlight other concomitant etiological factors. The possible hereditary or genetic component is most recently being explored, although facing tremendous dynamical elements to substantially delineate its role. The purpose of this review was to explore, reflect and call attention to the current knowledge of the genetic involvement of dental treatment fear and anxiety. Although findings mostly stress the relation between dental fear to other phenotypes giving rise to individual vulnerability, there are studies disclosing suggestive heritability rates for dental fear and anxiety.
- Hypohidrotic ectodermal dysplasia and its manifestations in the oral cavityPublication . Cardoso, Jennifer; de Faria Carvalho, Célio Domingos; Carvalho Silva, Cátia; Moura Teles, Ana; C. Leal, M. Fernanda; Lopes Cardoso, InêsThe Ectodermal Dysplasias generally present orofacial manifestations, such as skeletal discrepancies and dental alterations. Therefore, the role of a paediatric dentist in the detection and recognition of these repercussions can be crucial in early diagnosis of the disease. The oral rehabilitation of paediatric patients with this condition is extremely important, ideally, at a very early stage, yet contributing for the re-establishment of normal chewing, swallowing and phonetics functions, and, naturally, aesthetics increase. The purpose of this narrative review aims to elucidate dentists about their role in the detection, diagnosis, treatment and monitoring of the Ectodermal Dysplasia’ oral manifestations in paediatric patients, through the presentation of general physical and specific craniofacial characteristics.
- Intracanal medication as antibiotic control in case of permanent mature teeth with necrotic pulp or apical periodontitisPublication . Moura Teles, Ana; Lassarat, Rebecca; Pina, Cristina; Lopes Cardoso, InêsNon-Surgical Endodontic Treatment consists in the removal of dental pulp and the elimination of microorganisms that cause infection of the pulp space. This treatment can be performed in a single or in multiple sessions and, sometimes, the use of Intracanal Medication may be necessary. The purpose of Intracanalar Medication is to reach bacteria present in places inaccessible to instrumentation or irrigants and, also, to inhibit the invasion of the root canal system by bacteria or its products by coronal, lateral or apical communications with the outside through dentinal tubules, apical foramen or accessories root canals. The present work is a narrative review of the Literature that aims to analyse the different opinions among authors, in the case of permanent mature teeth with diagnosis of necrosis with or without apical periodontitis associated, and to understand in which situations it is necessary to apply Intracanal Medication, which should be chosen and how it should be applied. In this way, it is also intended that this work can be a useful guide for clinicians.
- Mental health and alcohol consumption among university students in the post-pandemic context: an exploratory cross-sectional study in PortugalPublication . Moreira, Teresa; Guimarães, Maria Inês; Silveira, Augusta; Loibl, Beatriz; Guedes Lopes, Beatriz; Alves Ribeiro Ferraz, Hugo Filipe; Castro, Inês; Mira de Almeida, Sofia; Lopes Cardoso, Inês; Pereira Rodrigues, Sandra Beatriz; Lima, Andreia Maria NovoIntroduction: The COVID-19 pandemic had significant effects on mental health and lifestyle behaviours, especially among university students who experienced academic disruptions, social isolation, and fewer social interactions. Alcohol consumption has long been part of student culture. Still, the influence of post-pandemic academic reintegration on drinking patterns and psychological distress remains relatively unexplored, particularly in countries like Portugal, where student traditions heavily shape consumption habits. This study aimed to describe the prevalence of alcohol consumption, depression, anxiety, and stress in a sample of Portuguese university students during the post-pandemic academic period, and to explore associations with sociodemographic variables. Methods: A cross-sectional study was conducted in November 2021 with 90 students from a private higher education institution in northern Portugal. Data were collected via an online questionnaire including sociodemographic information, the Alcohol Use Disorders Identification Test (AUDIT), and the Depression, Anxiety and Stress Scale (DASS-21). Result: The majority of the participants were not at risk of alcohol addiction (95.3%). In total, 15.1% of students reported anxiety symptoms ranging from severe to extremely severe. A binomial logistic regression was performed to ascertain the effects of being away from home and psychological distress (DASS-42 score), on the likelihood that participants were at risk of alcohol addiction (Level 3 and 4 in the AUDIT scale). The logistic regression model was statistically significant, χ2(2) = 9.20, p = 0.010. Living away from home was associated with a substantially lower likelihood of high-risk status (B = −2.79, p = 0.034), corresponding to an odds ratio of 0.06, indicating a strong protective effect. DASS-42 total score was positively associated with high-risk status (B = 0.04, p = 0.039), such that higher psychological distress increased the odds of being classified as high risk. Conclusions: The findings reveal a low prevalence of alcohol risk but heightened symptoms of anxiety, depression, and stress. Psychological distress notably increases the likelihood of hazardous alcohol use, emphasising the importance of targeted mental health and alcohol-use interventions among university students.
- Non-pharmacological interventions for anxiety relief in patients undergoing dental care and dental procedures: a scoping review protocolPublication . Oliveira, Lídia; Guimarães, Maria Inês; Lopes Cardoso, Inês; Moreira, TeresaIntroduction: Dental anxiety is a significant challenge in oral healthcare, affecting patients of all ages and potentially compromising treatment adherence, cooperation during procedures, and overall clinical outcomes. Non-pharmacological interventions have increasingly been used as complementary strategies to reduce anxiety and promote patient comfort during dental care and procedures. Objectives: To identify and map the existing scientific evidence on non-pharmacological interventions used to reduce anxiety in patients undergoing dental care and procedures. Methodology: A scoping review will be conducted in accordance with the Joanna Briggs Institute methodological guidance and reported in line with the PRISMA-ScR recommendations. The literature search will be conducted in PubMed, MEDLINE Complete, CINAHL Ultimate, Scopus, Psychology and Behavioral Sciences Collection, RCAAP, and BASE. Search strategies will combine controlled vocabulary, where applicable, with free-text terms structured around three conceptual blocks: anxiety or dental fear, dental/oral healthcare and procedures, and non-pharmacological interventions. Quantitative, qualitative, mixed-methods, observational, experimental, quasi-experimental, descriptive, and case studies meeting the eligibility criteria will be considered for inclusion. Study selection and data extraction will be conducted independently by two reviewers, with disagreements resolved through discussion and, when necessary, consultation with a third reviewer. The study selection process will be documented using a PRISMA-ScR flow diagram. The protocol has been registered and made publicly available through the Open Science Framework 10.17605/OSF.IO/J59PG. Expected Results: The review is expected to identify and categorise the range and characteristics of non-pharmacological interventions investigated for anxiety management during dental care and procedures, including complementary and holistic approaches, and to map the outcomes assessed and gaps in the existing evidence. Conclusion: This scoping review is expected to provide a comprehensive mapping of non-pharmacological strategies for anxiety management in dental care, supporting evidence-informed, patient-centred practice and identifying priorities for future research.
- Perception of Portuguese and Brazilian dentists regarding the importance of teeth and dental materials after exposure to high temperatures: forensics perspectivePublication . Ramos, Beatriz; Abreu, Isabel; Moreira, Teresa; Silveira, Augusta; Lopes Cardoso, Inês; Guimarães, Maria Inês; Mele, Federica; Santoro, ValeriaBackground: The main objective of this study was to carry out an online questionnaire in order to assess dentists’ knowledge and perception of dental materials submitted to high temperatures, helping to clarify and raise awareness of possible gaps in the area of forensic dentistry, especially in human identification. Results: Out of a total of 272 respondents, there was a greater influx of responses from countries such as Portugal and Brazil, the majority being female (66.3%), with an average age of 39 years. Most dentists consider complete clinical records to be extremely important. In addition, professionals with less experience provide fewer models in releasing their clinical records. In the case of dental materials, dentists consider composite resin and glass ionomers to be more susceptible to changes in shape and colour, while amalgam is considered the most stable, followed by ceramic and metal-ceramic crowns. On the other hand, dentin is considered more susceptible to colour changes than enamel. Enamel is seen by most professionals as resistant, undergoing few changes in shape at high temperatures. Conclusions: The opinion of the surveyed dentists on this subject is broadly in line with the existing literature. The only observed differences were related to exposure time and enamel resistance, since they assume that enamel is more resistant, and dentin is more vulnerable.