Percorrer por autor "Tramontana, Antea"
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- Degree of contamination of Gutta-Percha points by Staphylococcus aureus (MRSA/MSSA) strainsPublication . Moura Teles, Ana; Pina, Cristina; Lopes Cardoso, Inês; Tramontana, Antea; Cardoso, Miguel; Duarte, Ana Sofia; Bartolomeu, Maria; Noites, RitaMethicillin-resistant Staphylococcus aureus (MRSA) is considered one of the most harmful bacteria to human health. Dentistry, like all healthcare disciplines, places great emphasis on preventing scenarios that may result in cross-infection. Although various tested and already used materials are suitable for filling the root canal system, Gutta-Percha (GP) remains the preferred and widely accepted gold standard. Objective: We performed an in vitro analysis of the contamination of GP points, regarding the strains of Methicillin-resistant (MRSA) and Methicillin-sensitive (MSSA) Staphylococcus aureus, using classical microbiology methods and molecular biology techniques. Methods: Gutta-Percha points of two different brands from opened packages (already in use for 1 month) were collected for analysis. The assessment involved incubating the GP points in Brain Heart Infusion (BHI) medium to detect microbial growth. Growing microorganisms were plated on a selective and differential chromogenic medium for MRSA/MSSA strains, and the identification of isolates was confirmed by Polymerase Chain Reaction (PCR). In the case of microbial growth, the GP point was submitted to a disinfection protocol. Results: From the 315 collected GP points, only 6 (1.9%) resulted in being positive for microbial growth. After confirmation by PCR, only one sample of the six GP points was contaminated by MRSA, and the remaining five were MSSA-contaminated. The disinfection protocol was effective in all contaminated GP points. Conclusions: The Gutta-Percha points from opened pre-sterilized packages showed a very low degree of contamination by MRSA/MSSA. However, the detection of MSSA and MRSA strains raises concerns about potential contamination in dental clinic environments, and this risk cannot be considered negligible.
- Detection of the degree of contamination of Gutta-Percha points by strains of staphylococcus aureus (MRSA/MSSA)Publication . Tramontana, Antea; Pina, Cristina; Cardoso, Inês LopesMethicillin-Resistant Staphylococcus aureus (MRSA) is a bacteria considered one of the most harmful to human health. Dentistry is, as in all Health fields, extremely careful in avoiding situations that can lead to infection. Several tested materials suitable for filling the root canal system are available, still being gutta-percha the gold standard. Objective: Analysis in vitro of the contamination of gutta-percha points, regarding the bacteria Methicillin-Resistant (MRSA) and Methicillin-Sensitive (MSSA) Staphylococcus aureus, by classical microbiology methods and molecular biology techniques. Method: 315 gutta-percha points of two different brands from opened packed, already in use for 1 month were analysed, in Brain Heart Infusion medium to check the presence of microbial growth. Positive results were plated on a chromogenic selective medium for MRSA/MSSA strains, and the strains were confirmed by Polymerase Chain Reaction (PCR). In case of microbial growth, the gutta-percha point was submitted to a disinfection protocol. Results: From the 315 collected gutta-percha points, only 6 (1.9%) resulted microbial growth positive. After confirmation by PCR, only 1 sample of the 6 cones was a MRSA contamination, and the remaining 5 cones were MSSA. The disinfection protocol was effective in all of the contaminated GP points. Conclusions: Gutta-percha points from opened presterilized packed did show very little amount of contamination by MRSA/MSSA. However, the accurate handling of this filling material must be kept in mind soon since the pre-graduation period, as this methodology should be the gold standard for all professional activity.