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Identification of yeast and non-pigmented cultivable endodontic bacteria in adult portuguese patients

2013Book part Open access
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dc.contributor.authorTeles, Ana Moura
dc.contributor.authorManso, M. Conceição
dc.contributor.authorLoureiro, Sara Manuela Marinho
dc.contributor.authorSilva, Ricardo
dc.contributor.authorMadeira, Inês
dc.contributor.authorPina, Cristina Maria San Román Gomes de
dc.contributor.authorCabeda, José Manuel
dc.date.accessioned2021-02-23T01:19:10Z
dc.date.available2021-02-23T01:19:10Z
dc.date.issued2013
dc.date.updated2021-02-22T10:35:24Z
dc.description.abstractIntroduction This study has focused on the identification of the yeasts and non-pigmented bacteria present on adult patients with necrosis or apical periodontitis and the ones who resisted chemomechanical preparation and intracanal dressing with calcium hydroxide paste (Ca(OH)2) or 2% chlorohexidine digluconate gel (CHX). Methods 69 single-rooted teeth of adult patients with necrosis associated or not with apical periodontitis were selected (strict inclusion criteria); CHX group: 34 teeth; Ca(OH)2 group: 35 teeth. Bacteria samples were taken at baseline (S1), after chemo-mechanical preparation (S2) and after 14 days of intracanal dressing (S3). Bacteria and fungal presence was evaluate by means of culture in three atmospheres (aerobic, anaerobic, microaerofilic) in appropriate culture broads. Strict techniques were used for serial dilution, plating, incubation and identification. Results The most represented, abundant and prevalent strains of non-pigmented bacteria were Propionibacterium acnes (detected in S1, S2 and S3), Gemella morbillorum and Clostridium difficile. Candida albicans was found in 9 patients. The higher number of isolates proceeded from S1, being S2 the moment with lowest number of isolates. CHX had a worst performance in disinfection of the root canal system; consequently the number of isolates from S3 samples was bigger compared to Ca(OH)2. The number of identified bacterial species per canal/moment of sampling, varied from zero till 5, including yeasts (Candida albicans). Conclusions: Our findings confirm that the microbiota from primary endodontic infections is polymicrobial, and the anaerobes Gram-positive non-pigmented bacteria are well represented. CHX performed worse, consequently the number of isolates from S3 samples was bigger when compared to Ca(OH)2 as well as with diagnosis of necrosis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.isbn9788494213410
dc.identifier.slugcv-prod-2120091
dc.identifier.urihttp://hdl.handle.net/10284/9479
dc.language.isoengpt_PT
dc.publisherFormatex Research Centerpt_PT
dc.subjectEndodonticspt_PT
dc.subjectCulturept_PT
dc.subjectRoot Canal Samplingpt_PT
dc.titleIdentification of yeast and non-pigmented cultivable endodontic bacteria in adult portuguese patientspt_PT
dc.typebook part
dspace.entity.typePublication
oaire.citation.conferencePlaceEspanhapt_PT
oaire.citation.endPage1685pt_PT
oaire.citation.startPage1680pt_PT
oaire.citation.titleMicrobial pathogens and strategies for combating them: science, technology and educationpt_PT
person.familyNameManso
person.familyNameSilva
person.familyNameSan Román Gomes de Pina
person.familyNameManuel Baptista Cabeda
person.givenNameMaria da Conceição
person.givenNameRicardo
person.givenNameCristina Maria San Román Gomes de Pina
person.givenNameJosé
person.identifier.ciencia-id5A18-502F-4844
person.identifier.ciencia-idBA1E-FA67-80E5
person.identifier.ciencia-id5D11-7378-444A
person.identifier.ciencia-id3F17-D327-35C3
person.identifier.orcid0000-0002-1774-1337
person.identifier.orcid0000-0002-7276-4435
person.identifier.orcid0000-0002-3880-4712
person.identifier.orcid0000-0002-5117-2326
person.identifier.ridD-7212-2013
person.identifier.ridK-5451-2012
person.identifier.scopus-author-id57196702148
person.identifier.scopus-author-id7201604712
rcaap.cv.cienciaidB511-73B3-087A | Ana Moura Teles
rcaap.rightsopenAccesspt_PT
rcaap.typebookPartpt_PT
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relation.isAuthorOfPublication.latestForDiscoveryb24b5ee0-1368-472b-86b4-f5c743118a65

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