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Urinary volatilomics unveils a candidate biomarker panel for noninvasive detection of clear cell renal cell carcinoma

2021Journal article Restricted access
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dc.contributor.authorPinto, Joana
dc.contributor.authorAmaro, Filipa
dc.contributor.authorLima, Ana Rita
dc.contributor.authorCarvalho-Maia, Carina
dc.contributor.authorJerónimo, Carmen
dc.contributor.authorHenrique, Rui
dc.contributor.authorBastos, Maria de Lourdes
dc.contributor.authorCarvalho, Márcia
dc.contributor.authorGuedes de Pinho, Paula
dc.date.accessioned2021-06-30T09:42:37Z
dc.date.available2021-06-30T09:42:37Z
dc.date.issued2021
dc.description.abstractClear cell renal cell carcinoma (ccRCC) is the most common type of kidney cancer usually associated with asymptomatic development and risk of systemic progression. Hence, reliable molecular biomarkers of ccRCC are needed to provide early and minimally invasive detection. In this study, urinary volatilome profiling of patients diagnosed with ccRCC (n = 75), and cancer-free controls (n = 75), was performed to investigate the presence of a volatile signature characteristic of ccRCC. Volatile organic compounds (VOCs) in general, and more specifically volatile carbonyl compounds (VCCs), present in urine were extracted by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS). Supervised multivariate models showed a good discriminatory power of ccRCC patients from controls in urine. Overall, 22 volatile metabolites were found significantly altered between the two groups, including aldehydes, ketones, aromatic hydrocarbons, and terpenoids. A candidate six-biomarker panel, comprising octanal, 3-methylbutanal, benzaldehyde, 2-furaldehyde, 4-heptanone, and p-cresol, depicted the best performance for ccRCC detection with 83% sensitivity, 79% specificity, and 81% accuracy. Moreover, the ccRCC urinary volatilome signature suggested dysregulation of energy metabolism and overexpression of enzymes associated with carcinogenesis. These findings provide the molecular basis for the fine-tuning of gas-sensing materials for application in the development of a bioelectronic sensor.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1021/acs.jproteome.0c00936pt_PT
dc.identifier.eissn1535-3907
dc.identifier.issn1535-3893
dc.identifier.urihttp://hdl.handle.net/10284/9977
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherACS Publicationspt_PT
dc.relationThis work was financed by FEDER-Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020-Operacional Programme for Competitiveness and Internationalization (POCI), Portuguese funds through FCT- Fundação para a Ciência e a Tecnologia in the framework of the project POCI-01-0145-FEDER-030388−PTDC/SAU- SER/30388/2017.pt_PT
dc.subjectClear cell renal cell carcinomapt_PT
dc.subjectDiagnostic biomarkerspt_PT
dc.subjectUrinept_PT
dc.subjectVolatilomept_PT
dc.subjectGas chromatography - mass spectrometrypt_PT
dc.titleUrinary volatilomics unveils a candidate biomarker panel for noninvasive detection of clear cell renal cell carcinomapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3077pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage3068pt_PT
oaire.citation.titleJournal of Proteome Researchpt_PT
oaire.citation.volume20pt_PT
person.familyNameCarvalho
person.givenNameMarcia
person.identifier2017111
person.identifier.ciencia-id8B10-171E-E63E
person.identifier.orcid0000-0001-9884-4751
person.identifier.ridD-5999-2013
person.identifier.scopus-author-id7201413997
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3837b828-ba57-47f7-a811-cce65e4922c6
relation.isAuthorOfPublication.latestForDiscovery3837b828-ba57-47f7-a811-cce65e4922c6

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