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Determination of sexual dimorphism through molecular methods: a scoping review

2025Recensão Acesso aberto
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datacite.subject.fosCiências Médicas
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorGuimarães, Maria Inês
dc.contributor.authorMoreira, Teresa
dc.contributor.authorDupuis, Clarisse
dc.contributor.authorLopes Cardoso, Inês
dc.date.accessioned2026-05-11T11:43:50Z
dc.date.available2026-05-11T11:43:50Z
dc.date.issued2025
dc.description.abstractBackground: Sexual dimorphism is crucial in forensic investigations. Molecular methods involving amelogenin, a protein in tooth enamel, are used to determine sexual dimorphism by extracting DNA from teeth, amplifying the gene coding for amelogenin through PCR, and analyzing PCR product sizes to identify X and/or Y chromosomes. Objective: This scoping review explored scientific studies using the amelogenin gene to determine sex in forensic dentistry. It addressed the research question: do molecular methods determine sexual dimorphism for forensic identification? Material and methods: A literature review (1996–2024) was conducted using PubMed, MEDLINE (via BVS), and CINAHL (via EBSCO host). Inclusion and exclusion criteria guided the selection process, summarized in a PRISMA flowchart. A PCC (Population-Concept-Context) strategy was applied to formulate the research question. Results: Thirteen of the 1091 articles initially considered met the inclusion and exclusion criteria. These studies examined forensic dentistry and sex determination through amelogenin identification. They were categorized into “with treatment” (7 studies) and “without treatment” (6 studies), and all involved in vitro research. Conclusion: Molecular methods targeting the amelogenin gene on the X and Y chromosomes offer an accurate and reliable approach to determining sex.eng
dc.identifier.citationGuimarães, M. I., Moreira, M. T., Dupuis, C., & Cardoso, I. L. (2025). Determination of sexual dimorphism through molecular methods: A scoping review. Egyptian Journal of Forensic Sciences, 15, 11. https://doi.org/10.1186/s41935-025-00432-6
dc.identifier.doi10.1186/s41935-025-00432-6
dc.identifier.issn2090536X, 20905939
dc.identifier.urihttp://hdl.handle.net/10284/15413
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Open
dc.relation.hasversionhttps://link.springer.com/article/10.1186/s41935-025-00432-6
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectForensic dentistry
dc.subjectAnalyses
dc.subjectSex determination
dc.subjectAmelogenin
dc.titleDetermination of sexual dimorphism through molecular methods: a scoping reviewpor
dc.typereview
dspace.entity.typePublication
oaire.citation.issue11
oaire.citation.titleEgyptian Journal of Forensic Sciences
oaire.citation.volume15
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGuimarães
person.familyNameMoreira
person.familyNameDupuis
person.familyNameLopes Cardoso
person.givenNameMaria Inês
person.givenNameTeresa
person.givenNameClarisse
person.givenNameInês
person.identifier2532024
person.identifier.ciencia-id5416-2525-4C8B
person.identifier.ciencia-idFD12-7090-7D84
person.identifier.ciencia-idF21F-16B4-3715
person.identifier.orcid0000-0003-3687-7798
person.identifier.orcid0000-0002-2554-697X
person.identifier.orcid0009-0009-6436-9441
person.identifier.orcid0000-0002-0693-9831
person.identifier.ridM-7156-2013
person.identifier.scopus-author-id57213512502
person.identifier.scopus-author-id24073457200
person.identifier.scopus-author-id54973754300
relation.isAuthorOfPublication54d88a9d-f83b-4cce-8cc5-aac9ed7e701b
relation.isAuthorOfPublication50d43aef-4063-463c-abee-deb7ac2b2853
relation.isAuthorOfPublication563b7779-9b02-43ac-b029-00adb8107aa1
relation.isAuthorOfPublicationf39fa18f-2d23-4690-bfa2-f8ba53e40775
relation.isAuthorOfPublication.latestForDiscoveryf39fa18f-2d23-4690-bfa2-f8ba53e40775

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