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Effects of over-expression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseus

dc.contributor.authorCanel, Camilo
dc.contributor.authorCardoso, Inês Lopes
dc.contributor.authorWhitmer, Serap
dc.contributor.authorFits, Leslie Van Der
dc.contributor.authorPasquali, Giancarlo
dc.contributor.authorHeijden, Robert van der
dc.contributor.authorHoge, J. Harry C.
dc.contributor.authorVerpoorte, Robert
dc.date.accessioned2020-03-05T18:21:33Z
dc.date.available2020-03-05T18:21:33Z
dc.date.issued1998
dc.date.updated2020-02-28T16:55:10Z
dc.description.abstractCells of Catharanthus roseus (L.) G. Don were genetically engineered to over-express the enzymes strictosidine synthase (STR; EC 4.3.3.2) and tryptophan decarboxylase (TDC; EC 4.1.1.28), which catalyze key steps in the biosynthesis of terpenoid indole alkaloids (TIAs). The cultures established after Agrobacteriummediated transformation showed wide phenotypic diversity, re¯ecting the complexity of the biosynthetic pathway. Cultures transgenic for Str consistently showed tenfold higher STR activity than wild-type cultures, which favored biosynthetic activity through the pathway. Two such lines accumulated over 200 mg á L)1 of the glucoalkaloid strictosidine and/or strictosidine-derived TIAs, including ajmalicine, catharanthine, serpentine, and tabersonine, while maintaining wild-type levels of TDC activity. Alkaloid accumulation by highly productive transgenic lines showed considerable instability and was strongly in¯uenced by culture conditions, such as the hormonal composition of the medium and the availability of precursors. High transgene-encoded TDC activity was not only unnecessary for increased productivity, but also detrimental to the normal growth of the cultures. In contrast, high STR activity was tolerated by the cultures and appeared to be necessary, albeit not su cient, to sustain high rates of alkaloid biosynthesis. We conclude that constitutive over-expression of Str is highly desirable for increased TIA production. However, given its complexity, limited intervention in the TIA pathway will yield positive results only in the presence of a favorable epigenetic environment.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.slugcv-prod-368669
dc.identifier.urihttp://hdl.handle.net/10284/8620
dc.language.isoengpt_PT
dc.subjectCell culturept_PT
dc.subjectGenetic engineeringpt_PT
dc.subjectSecondary metabolismpt_PT
dc.subjectStrictosidine synthasept_PT
dc.subjectTerpenoid indole alkaloidspt_PT
dc.subjectTryptophan decarboxylasept_PT
dc.titleEffects of over-expression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlacePaíses Baixospt_PT
oaire.citation.endPage419pt_PT
oaire.citation.startPage414pt_PT
oaire.citation.titlePlantapt_PT
oaire.citation.volume205pt_PT
person.familyNameLopes Cardoso
person.givenNameInês
person.identifier.ciencia-idF21F-16B4-3715
person.identifier.orcid0000-0002-0693-9831
person.identifier.ridM-7156-2013
person.identifier.scopus-author-id54973754300
rcaap.cv.cienciaidF21F-16B4-3715 | Maria Inês de Avelar Lopes Cardoso
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf39fa18f-2d23-4690-bfa2-f8ba53e40775
relation.isAuthorOfPublication.latestForDiscoveryf39fa18f-2d23-4690-bfa2-f8ba53e40775

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