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Implementation of HPLC methodology for the quantification of malondialdehyde in cell suspensions and liver

2004Journal article Restricted access
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dc.contributor.authorSoares, M. Elisa
dc.contributor.authorCarvalho, Márcia
dc.contributor.authorRemião, Fernando
dc.contributor.authorCarvalho, Félix
dc.contributor.authorBastos, Maria de Lourdes
dc.date.accessioned2021-07-01T15:51:42Z
dc.date.available2021-07-01T15:51:42Z
dc.date.issued2004
dc.description.abstractDetermination of malondialdehyde (MDA) is commonly used for the evaluation of lipid peroxidation in biological human samples, especially in plasma, serum, and urine, as a non‐invasive biomarker of some oxidative‐stress‐related diseases. The objective of the present study was to implement a HPLC methodology for the quantification of MDA in cell suspensions and liver, after complexing this compound with thiobarbituric acid (TBA), giving rise to the formation of the complex MDA–(TBA)2. The implemented methodology was validated and allowed a reliable separation and quantification of MDA, with high precision and recovery, and a low detection limit. The stability of MDA in the biological samples, as well as of the MDA–(TBA)2 complex, was also evaluated during storage at different temperatures and for different time periods. The data obtained from the stability studies concerning the MDA–(TBA)2 complex prepared from hepatocyte suspensions and liver samples, indicated that the chromatographic analysis should be performed within the period of 4 hr when it is maintained at ambient temperature or at 40°C, and within the period of 48 hr if the temperatures of the storage are 4°C, −20°C, or −80°C. When the biological samples are conserved for posterior preparation of the complex, MDA can be measured in the cell suspensions conserved at any of these temperatures in the subsequent 24 hr, but liver samples must be conserved at 4°C or −20°C in order to obtain reliable results of MDA.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1081/JLC-200028139pt_PT
dc.identifier.issn1082-6076
dc.identifier.issn1520-572X
dc.identifier.urihttp://hdl.handle.net/10284/10004
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relationThis work was supported by a FCT/POCTI/FEDER European Community project (POCTI/36099/FCB/2000).pt_PT
dc.subjectMalondialdehydept_PT
dc.subjectHPLC/UVpt_PT
dc.subjectCell suspensionspt_PT
dc.subjectLiverpt_PT
dc.titleImplementation of HPLC methodology for the quantification of malondialdehyde in cell suspensions and liverpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2369pt_PT
oaire.citation.issue15pt_PT
oaire.citation.startPage2357pt_PT
oaire.citation.titleJournal of Liquid Chromatography & Related Technologiespt_PT
oaire.citation.volume27pt_PT
person.familyNameCarvalho
person.givenNameMarcia
person.identifier2017111
person.identifier.ciencia-id8B10-171E-E63E
person.identifier.orcid0000-0001-9884-4751
person.identifier.ridD-5999-2013
person.identifier.scopus-author-id7201413997
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3837b828-ba57-47f7-a811-cce65e4922c6
relation.isAuthorOfPublication.latestForDiscovery3837b828-ba57-47f7-a811-cce65e4922c6

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