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Noninvasive methods to determine the critical micelle concentration of some bile acid salts

2004Journal article Open access
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dc.contributor.authorReis, Salette
dc.contributor.authorMoutinho, Carla Guimarães
dc.contributor.authorMatos, Carla
dc.contributor.authorde Castro, Baltazar
dc.contributor.authorGameiro, Paula
dc.contributor.authorLima, José L.F.C.
dc.date.accessioned2019-12-24T10:11:42Z
dc.date.available2019-12-24T10:11:42Z
dc.date.issued2004
dc.description.abstractIn this work the critical micelle concentrations (cmc) of four bile salts, sodium cholate, sodium glycocholate, sodium deoxycholate, and sodium glycodeoxycholate, are determined and presented. Three independent noninvasive methodologies (potentiometry, derivative spectrophotometry, and light scattering) were used for cmc determination, at 25 degrees C with ionic strength adjusted to 0.10 M with NaCl. Spectrophotometric and potentiometric studies of some bile salts were also executed at various ionic strength values, thus allowing the influence of the ionic strength on the cmc value of the bile salt to be assessed. A critical comparison of the cmc values obtained with data collected from the literature is presented. Furthermore, this work makes an evaluation of the conceptual bases of different methodologies commonly used for cmc determination, since variations in the results obtained can be related mainly to different intrinsic features of the methods used (such as sensitivity or the need to include tracers or probes) or to the operational cmc definition applied. The undoubted definition of the experimental bile salt concentration that corresponds to cmc (operational cmc) is essential since in the case of these amphiphiles the formation of micelles is not as abrupt as in the case of ordinary association colloids. The biphasic nature of their aggregation leads to a "round-shaped" variation of the experimental parameters under analysis, which makes difficult the evaluation of the cmc values and can be responsible for the different results obtained.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ab.2004.07.017pt_PT
dc.identifier.issn0003-2697
dc.identifier.issn1096-0309
dc.identifier.urihttp://hdl.handle.net/10284/8325
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier Massonpt_PT
dc.relationDevelopment and application of in vitro methodologies for evaluation of anti-inflammatory and antioxidant mechanisms of non-steroidal anti-inflammatory drugs
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0003269704006116pt_PT
dc.subjectBile Acids and Saltspt_PT
dc.subjectDeoxycholic Acidpt_PT
dc.subjectGlycocholic Acidpt_PT
dc.subjectGlycodeoxycholic Acidpt_PT
dc.subjectMethodspt_PT
dc.subjectMicellespt_PT
dc.subjectOsmolar Concentrationpt_PT
dc.subjectPhase Transitionpt_PT
dc.subjectPotentiometrypt_PT
dc.subjectSodium Cholatept_PT
dc.subjectSpectrum Analysispt_PT
dc.titleNoninvasive methods to determine the critical micelle concentration of some bile acid saltspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment and application of in vitro methodologies for evaluation of anti-inflammatory and antioxidant mechanisms of non-steroidal anti-inflammatory drugs
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FFCB%2F47186%2F2002/PT
oaire.citation.endPage126pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage117pt_PT
oaire.citation.titleAnalytical Biochemistrypt_PT
oaire.citation.volume334pt_PT
oaire.fundingStreamPOCI
person.familyNameMoutinho
person.familyNameMatos
person.givenNameCarla
person.givenNameCarla
person.identifier1536969
person.identifier.ciencia-idB61F-F356-80C6
person.identifier.ciencia-id721C-DA34-256E
person.identifier.orcid0000-0003-1310-4696
person.identifier.orcid0000-0003-0617-3538
person.identifier.ridM-7142-2013
person.identifier.scopus-author-id16064733800
person.identifier.scopus-author-id7004493818
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationcff7638e-1f33-4384-aa73-d3acc5cca5f8
relation.isAuthorOfPublicationd4b6d3e1-6d29-4fb4-97c7-abb2cf4c83b4
relation.isAuthorOfPublication.latestForDiscoveryd4b6d3e1-6d29-4fb4-97c7-abb2cf4c83b4
relation.isProjectOfPublication9bcf495f-7342-4ba2-b02d-bcfe49fd8b9f
relation.isProjectOfPublication.latestForDiscovery9bcf495f-7342-4ba2-b02d-bcfe49fd8b9f

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