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Structural and functional stabilization of phage particles in carbohydrate matrices for bacterial biosensing

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dc.contributor.authorBalcão, Victor
dc.contributor.authorMoreira, Ana R.
dc.contributor.authorMoutinho, Carla
dc.contributor.authorChaud, Marco V.
dc.contributor.authorTubino, Matthieu
dc.contributor.authorVila, Marta M.D.C.
dc.date.accessioned2019-12-24T11:52:42Z
dc.date.available2019-12-24T11:52:42Z
dc.date.issued2013
dc.description.abstractInfections associated with health care services are nowadays widespread and, associated to the progressive emergence of microorganisms resistant to conventional chemical antibiotics, are major causes of morbidity and mortality. One of the most representative microorganisms in this scenario is the bacterium Pseudomonas aeruginosa, which alone is responsible for ca. 13-15% of all nosocomial infections. Bacteriophages have been reported as a potentially useful tool in the diagnosis of bacterial diseases, since they specifically recognize and lyse bacterial isolates thus confirming the presence of viable cells. In the present research effort, immobilization of these biological (although metabolically inert) entities was achieved via entrapment within (optimized) porous (bio)polymeric matrices of alginate and agar, aiming at their full structural and functional stabilization. Such phage-impregnated polymeric matrices are intended for future use as chromogenic hydrogels sensitive to color changes evolving from reaction with (released) intracytoplasmatic moieties, as a detection kit for P. aeruginosa cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.enzmictec.2013.03.001pt_PT
dc.identifier.issn0141-0229
dc.identifier.issn1879-0909
dc.identifier.urihttp://hdl.handle.net/10284/8337
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0141022913000392pt_PT
dc.subjectAgarpt_PT
dc.subjectAlginatespt_PT
dc.subjectBacteriological Techniquespt_PT
dc.subjectBiopolymerspt_PT
dc.subjectBiosensing Techniquespt_PT
dc.subjectCells, Immobilizedpt_PT
dc.subjectCross Infectionpt_PT
dc.subjectGlucuronic Acidpt_PT
dc.subjectHexuronic Acidspt_PT
dc.subjectHumanspt_PT
dc.subjectHydrogelspt_PT
dc.subjectPseudomonas Infectionspt_PT
dc.subjectPseudomonas Phagespt_PT
dc.subjectPseudomonas aeruginosapt_PT
dc.titleStructural and functional stabilization of phage particles in carbohydrate matrices for bacterial biosensingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage69pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage55pt_PT
oaire.citation.titleEnzyme and Microbial Technologypt_PT
oaire.citation.volume53pt_PT
person.familyNameBalcão
person.familyNameMoutinho
person.givenNameVictor
person.givenNameCarla
person.identifier366700
person.identifier.ciencia-idC314-A18C-931B
person.identifier.ciencia-idB61F-F356-80C6
person.identifier.orcid0000-0003-0772-2834
person.identifier.orcid0000-0003-1310-4696
person.identifier.ridI-2182-2012
person.identifier.ridM-7142-2013
person.identifier.scopus-author-id6603449856
person.identifier.scopus-author-id16064733800
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf84a6560-46a5-4a51-91e4-25ab97dd1319
relation.isAuthorOfPublicationcff7638e-1f33-4384-aa73-d3acc5cca5f8
relation.isAuthorOfPublication.latestForDiscoverycff7638e-1f33-4384-aa73-d3acc5cca5f8

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