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Bacterial contamination of gutta-percha points from different brands and the efficacy of a chairside disinfection protocol

2020-11-09Journal article Open access
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dc.contributor.authorBracciale, Francesca
dc.contributor.authorMarino, Nicole
dc.contributor.authorNoronha, Anariely
dc.contributor.authorManso, M. Conceição
dc.contributor.authorGavinha, Sandra
dc.contributor.authorCardoso, Inês Lopes
dc.contributor.authorPina, Cristina Maria San Román Gomes de
dc.contributor.authorTeles, Ana Moura
dc.date.accessioned2021-02-17T23:36:43Z
dc.date.available2021-02-17T23:36:43Z
dc.date.issued2020-11-09
dc.date.updated2021-02-09T16:55:09Z
dc.description.abstractObjective: To evaluate the bacterial contamination of different brands of Gutta-Percha (GP) points routinely used in clinical practice and the efficacy of a chairside disinfection protocol with sodium hypochlorite. Methods: GP points (n=240), in sizes A, B, C, D, K15, K20, K25, K30, K35, K40, F1, F2, F3 (Dentsply®, Proclinic®, ProTaper® and R&S®), were randomly sampled from commercial packages already in use. These were added directly to Fluid Thioglycolate Medium (one GP point per tube) and incubated at 37ºC for 21 days. During this period, the presence/absence of turbidity was evaluated. To evaluate the efficacy of a chairside disinfection protocol, all detected contaminated GP points were immersed for 1 minute in 10 mL of 5.25% sodium hypochlorite, followed by 5 minutes in 10 mL of detergent solution (3% Tween 80 and 5% sodium thiosulfate) and a final rinse with 10 mL of sterile distilled water and incubated. The data was analysed using the chi-square test and differences between characteristics of dichotomic variables were performed using the binomial test. The significance level was set at P<0.05. Results: Bacterial growth was observed in 22.9% of the total study samples. Dentsply® and R&S® showed the highest level of contamination, 47.3% each, although without significant differences to the other commercial brands. The most contaminated GP point size was K30 (16.4%). The chairside disinfection protocol was effective in disinfection of 76.4% of GP points (P<0.001). Conclusion: A real small number of GP points in clinical use harboured bacteria, including after the Chairside Disinfection Protocol that, anyway, proved to be effective. No significant difference was observed between tested commercial brands.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBracciale, F., Marino, N., Noronha, A., Manso, M.C., Gavinha, S., Cardoso, I.L., Pina, C., Teles, A.M. (2020). Bacterial contamination of gutta-percha points from different brands and the efficacy of a chairside disinfection protocol. European Endodontic Journal. https://doi.org/10.14744/eej.2020.44265
dc.identifier.doi10.14744/eej.2020.44265pt_PT
dc.identifier.issn2548-0839
dc.identifier.slugcv-prod-2176168
dc.identifier.urihttp://hdl.handle.net/10284/9432
dc.language.isoengpt_PT
dc.publisherKare Publishingpt_PT
dc.subjectContaminationpt_PT
dc.subjectDisinfection protocolpt_PT
dc.subjectEndodontic treatmentpt_PT
dc.subjectGutta-percha pointspt_PT
dc.subjectRoot canal fillingpt_PT
dc.subjectSecondary endodontic infectionpt_PT
dc.titleBacterial contamination of gutta-percha points from different brands and the efficacy of a chairside disinfection protocolpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage287pt_PT
oaire.citation.startPage282pt_PT
oaire.citation.titleEuropean Endodontic Journalpt_PT
oaire.citation.volume3pt_PT
person.familyNameManso
person.familyNameGavinha
person.familyNameLopes Cardoso
person.familyNameSan Román Gomes de Pina
person.givenNameMaria da Conceição
person.givenNameSandra
person.givenNameInês
person.givenNameCristina Maria San Román Gomes de Pina
person.identifier.ciencia-id5A18-502F-4844
person.identifier.ciencia-id0A14-1FF4-3383
person.identifier.ciencia-idF21F-16B4-3715
person.identifier.ciencia-id5D11-7378-444A
person.identifier.orcid0000-0002-1774-1337
person.identifier.orcid0000-0002-2430-1241
person.identifier.orcid0000-0002-0693-9831
person.identifier.orcid0000-0002-3880-4712
person.identifier.ridD-7212-2013
person.identifier.ridM-7156-2013
person.identifier.scopus-author-id57196702148
person.identifier.scopus-author-id35758590200
person.identifier.scopus-author-id54973754300
rcaap.cv.cienciaidB511-73B3-087A | Ana Moura Teles
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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