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An assay for secologanin in plant tissues based on enzymatic conversion into strictosidine

1998Journal article Open access
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dc.contributor.authorHallard, Didier
dc.contributor.authorHeijden, Robert Van Der
dc.contributor.authorContin, Adriana
dc.contributor.authorTomaz Jiméréz, Emília M.
dc.contributor.authorSnoeijer, Wim
dc.contributor.authorVerpoorte, Robert
dc.contributor.authorJensen, Soren R.
dc.contributor.authorCardoso, M. Inês Lopes
dc.contributor.authorPasquali, Giancarlo
dc.contributor.authorMemelink, Johan
dc.contributor.authorHoge, J. Harry C.
dc.date.accessioned2020-03-05T18:51:45Z
dc.date.available2020-03-05T18:51:45Z
dc.date.issued1998
dc.date.updated2020-02-28T17:02:49Z
dc.description.abstractThe secoiridoid glucoside secologanin is the terpenoid building block in the biosynthesis of terpenoid indole alkaloids. A method for its determination in plant tissues and in cell suspension cultures has been developed. This assay is based on the condensation of secologanin with tryptamine, yielding strictosidine, in a reaction catalysed by the enzyme strictosidine synthase (STR; E.C. 4.3.3.2). Subsequently, the formation of strictosidine is quantified by high performance liquid chromatography (HPLC). STR was isolated from transgenic Nicotiana tabacum cells expressing a cDNA-derived gene coding for STR from Catharanthus roseus. The high specificity of STR for secologanin, in combination with a sensitive and selective HPLC system, allows a simple extraction of secologanin from plant tissue. The detection limit of this method is 15 ng secologanin. Using this assay, secologanin contents were determined in tissues of various plant species; Lonicera xylosteum hairy roots were found to contain 1% of secologanin on a dry weight basis. # 1998 John Wiley & Sons, Ltd.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHallard, D., Heijden, R. v. d., Contin, A., Jiménéz, E. M. T., Snoeijer, W., Verpoorte, R., Jensen, S. R., Cardoso, M. I. L., Pasquali, G., Memelink, J., & Hoge, J. H. C. (1998). An assay for secologanin in plant tissues based on enzymatic conversion into strictosidine. Phytochemical Analysis, 9(4), 162–167. https://doi.org/10.1002/(sici)1099-1565(199807/08)9:4%3C162::aid-pca394%3E3.0.co;2-d
dc.identifier.slugcv-prod-368688
dc.identifier.urihttp://hdl.handle.net/10284/8624
dc.language.isoengpt_PT
dc.subjectNicotiana tabacumpt_PT
dc.subjectHigh performance liquid chromatographypt_PT
dc.subjectStrictosidine synthasept_PT
dc.subjectSecoiridoidspt_PT
dc.subjectSecologaninpt_PT
dc.titleAn assay for secologanin in plant tissues based on enzymatic conversion into strictosidinept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage167pt_PT
oaire.citation.startPage162pt_PT
oaire.citation.titlePhytochemical Analysispt_PT
oaire.citation.volume9pt_PT
person.familyNameLopes Cardoso
person.givenNameInês
person.identifier.ciencia-idF21F-16B4-3715
person.identifier.orcid0000-0002-0693-9831
person.identifier.ridM-7156-2013
person.identifier.scopus-author-id54973754300
rcaap.cv.cienciaidF21F-16B4-3715 | Maria Inês de Avelar Lopes Cardoso
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf39fa18f-2d23-4690-bfa2-f8ba53e40775
relation.isAuthorOfPublication.latestForDiscoveryf39fa18f-2d23-4690-bfa2-f8ba53e40775

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