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Peroxidase and the biosynthesis of terpenoid indole alkaloids in the medicinal plant Catharanthus roseus (L.) G. Don

2004Journal article Restricted access
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dc.contributor.authorSottomayor, M.
dc.contributor.authorCardoso, Inês Lopes
dc.contributor.authorPereira, L.G.
dc.contributor.authorRos Barceló, A.
dc.date.accessioned2020-03-05T19:18:47Z
dc.date.available2020-03-05T19:18:47Z
dc.date.issued2004
dc.date.updated2020-02-28T17:14:46Z
dc.description.abstractThe leaves of Catharanthus roseus (L.) G. Don produce the first natural drugs used in cancer therapy – the dimeric terpenoid indole alkaloids vinblastine and vincristine. The study of C. roseus further revealed two other terpenoid indole alkaloids with important pharmacological activity: ajmalicine, used as an antihypertensive, and serpentine, used as sedative. The biosynthetic pathway of the medicinal alkaloids has been investigated in much detail and a number of steps are now well characterized at the enzyme and gene level and, recently, several regulatory genes have also been isolated and characterized. Since early studies of the biosynthesis of vinblastine, during the 1970s and 1980s, the dimerization reaction has attracted much attention due to its possible regulatory importance and potential application for the semi synthetic production of the dimeric alkaloids. After initial, inconclusive work suggesting the involvement of peroxidase-like enzymes, the search for the dimerization enzyme in leaf tissue detected a single dimerization activity credited to the single class III plant peroxidase present in the leaves of the plant – the basic isoenzyme CRPRX1. The enzyme was purified to homogeneity, the respective cDNA and genomic sequences were characterized, and a channeling mechanism was proposed for the peroxidase-mediated-vacuolar synthesis of the first dimeric alkaloid intermediate, α-3 ,4 -anhydrovinblastine. On the other hand, the oxidation of ajmalicine into serpentine has been attributed to basic peroxidase isoenzymes localized in the vacuole of C. roseus cells. An overview of the work implying class III plant peroxidases in the biosynthesis of terpenoid indole alkaloids in C. roseus is presented here.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.slugcv-prod-368664
dc.identifier.urihttp://hdl.handle.net/10284/8627
dc.language.isoengpt_PT
dc.publisherKluwer Academic Publisherspt_PT
dc.subjectAjmalicinept_PT
dc.subjectBasic plant peroxidasespt_PT
dc.subjectCatharanthinept_PT
dc.subjectCatharanthus roseuspt_PT
dc.subjectCRPRX1pt_PT
dc.subjectSerpentinept_PT
dc.subjectVacuolept_PT
dc.subjectVindolinept_PT
dc.subjectα-3pt_PT
dc.subject4-anhydrovinblastinept_PT
dc.titlePeroxidase and the biosynthesis of terpenoid indole alkaloids in the medicinal plant Catharanthus roseus (L.) G. Donpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlacePaíses Baixospt_PT
oaire.citation.endPage171pt_PT
oaire.citation.startPage159pt_PT
oaire.citation.titlePhytochemistry Reviewspt_PT
oaire.citation.volume3pt_PT
person.familyNameLopes Cardoso
person.givenNameInês
person.identifier.ciencia-idF21F-16B4-3715
person.identifier.orcid0000-0002-0693-9831
person.identifier.ridM-7156-2013
person.identifier.scopus-author-id54973754300
rcaap.cv.cienciaidF21F-16B4-3715 | Maria Inês de Avelar Lopes Cardoso
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf39fa18f-2d23-4690-bfa2-f8ba53e40775
relation.isAuthorOfPublication.latestForDiscoveryf39fa18f-2d23-4690-bfa2-f8ba53e40775

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