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Protective effect of quince (Cydonia oblonga Miller) fruit against oxidative hemolysis of human erythrocytes

2009Journal article Restricted access
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dc.contributor.authorMagalhães, Ana S.
dc.contributor.authorSilva, Branca M.
dc.contributor.authorPereira, José A.
dc.contributor.authorAndrade, Paula B.
dc.contributor.authorValentão, Patrícia
dc.contributor.authorCarvalho, Márcia
dc.date.accessioned2021-07-02T14:08:17Z
dc.date.available2021-07-02T14:08:17Z
dc.date.issued2009
dc.description.abstractThe aim of this study was to determine the phenolic content and evaluate the antioxidant activity of quince (Cydonia oblonga) fruit. For this purpose, fruits were separated into pulps, peels and seeds and methanolic extracts were prepared. The phenolic profiles were determined by HPLC/UV and antioxidant properties were studied for their ability to quench the stable free radical 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis of human erythrocytes. The main phenolic compounds were 5-O-caffeoylquinic acid for pulp and peel (57% and 29%, respectively) and stellarin-2 for seed (18%). Total phenolics content was 2.5, 6.3 and 0.4g/kg of methanolic extract for pulp, peel and seed, respectively. Pulp and peel extracts showed similar DPPH free radical scavenging activities (EC(50) of 0.6 and 0.8 mg/ml, respectively), while seed extract presented much lower antioxidant potential (EC(50) of 12.2mg/ml). Under the oxidative action of AAPH, pulp and peel extracts showed significant protection of the erythrocyte membrane from hemolysis, in a time- and concentration-dependent manner. Seed extracts by themselves induced extensive hemolysis. These results indicate higher antioxidant activity for certain parts of quince fruit, namely pulp and peel, that may therefore represent accessible sources of natural antioxidants with potential application in nutritional/pharmaceutical fields, as preventive or therapeutic agents in diseases in which free radicals are implicated.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.fct.2009.03.017pt_PT
dc.identifier.issn0278-6915
dc.identifier.urihttp://hdl.handle.net/10284/10032
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectCydonia oblongapt_PT
dc.subjectQuincept_PT
dc.subjectPhenolic compoundspt_PT
dc.subjectErythrocytept_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectHemolysispt_PT
dc.titleProtective effect of quince (Cydonia oblonga Miller) fruit against oxidative hemolysis of human erythrocytespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1377pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage1372pt_PT
oaire.citation.titleFood and Chemical Toxicologypt_PT
oaire.citation.volume47pt_PT
person.familyNameSilva
person.familyNameCarvalho
person.givenNameBranca Maria
person.givenNameMarcia
person.identifier1033309
person.identifier2017111
person.identifier.ciencia-idBD1F-B24D-89A9
person.identifier.ciencia-id8B10-171E-E63E
person.identifier.orcid0000-0002-5610-8024
person.identifier.orcid0000-0001-9884-4751
person.identifier.ridD-5999-2013
person.identifier.scopus-author-id7005615589
person.identifier.scopus-author-id7201413997
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication26c68872-1849-4895-a48d-72ccffbc02df
relation.isAuthorOfPublication3837b828-ba57-47f7-a811-cce65e4922c6
relation.isAuthorOfPublication.latestForDiscovery26c68872-1849-4895-a48d-72ccffbc02df

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