Publication
Effectiveness of two intracanal dressings in adult portuguese patients: a qPCR and anaerobic culture assessment
| dc.contributor.author | Teles, Ana Moura | |
| dc.contributor.author | Manso, M. Conceição | |
| dc.contributor.author | Loureiro, S. | |
| dc.contributor.author | Silva, R. | |
| dc.contributor.author | Madeira, I. G. C. | |
| dc.contributor.author | Pina, C. | |
| dc.contributor.author | Cabeda, José Manuel | |
| dc.date.accessioned | 2021-02-23T00:29:00Z | |
| dc.date.available | 2021-02-23T00:29:00Z | |
| dc.date.issued | 2013 | |
| dc.date.updated | 2021-02-20T17:27:16Z | |
| dc.description.abstract | Aim: To quantify bacterial equivalents before and after chemomechanical preparation using 3% sodium hypochlorite (NaOCl) and intracanal dressing with calcium hydroxide paste (Ca(OH)2 ) or 2% Chlorhexidine digluconate gel (CHX) in necrotic pulps associated or not with apical periodontitis and to further compare this quantification with counts of anaerobic microorganisms. Methodology: Prospective clinical trial in 69 single-rooted adult teeth (strict inclusion criteria); CHX group: 34; Ca(OH)2 group: 35. Bacteria samples were taken at baseline (S1), after chemomechanical preparation (S2) and after 14 days of intracanal dressing (S3). Bacterial equivalents were assessed by broad-range real-time polymerase chain reaction (qPCR), and live viable bacteria measured with conventional anaerobic culture (CFU/mL). Descriptive/inferential analysis was performed with spss vs. 20.0 (α = 0.05) using the Kruskal-Wallis, Mann-Whitney and chi-squared tests and Spearman's correlation coefficients. Results: Both groups showed a significant decrease between S1 and S2 (Mann-Whitney U-test; P < 0.001) both in qPCR and in culture. In the Ca(OH)2 -group, no variation was observed between S2 and S3 by qPCR and culture. In contrast, the CHX group showed a significant increase from S2 to S3 by both techniques. The two groups were only significantly different in S3 (Mann-Whitney U-test; P ≤ 0.001), with a worse performance in the CHX group. Again, these results were congruent by both approaches. Data from both approaches correlate reasonably (rS < 0.5). Conclusions: Infected root canals contained a high bacterial load, and the chemomechanical root canal preparation reduced bacterial equivalents by 99.1% and anaerobic counts by 98.5%. Intracanal dressings were not efficient at reducing bacterial load, but the 14-day intracanal dressing with Ca(OH)2 performed significantly better than CHX, particularly in cases with apical periodontitis. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.1111/iej.12122 | pt_PT |
| dc.identifier.issn | 0143-2885 | |
| dc.identifier.issn | 1365-2591 | |
| dc.identifier.slug | cv-prod-2120088 | |
| dc.identifier.uri | http://hdl.handle.net/10284/9477 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | Wiley-Blackwell | pt_PT |
| dc.subject | Apical periodontitis | pt_PT |
| dc.subject | Endodontic microbiology | pt_PT |
| dc.subject | Intracanal dressing | pt_PT |
| dc.subject | qPCR | pt_PT |
| dc.subject | Real-time PCR | pt_PT |
| dc.subject | Root canal sampling | pt_PT |
| dc.title | Effectiveness of two intracanal dressings in adult portuguese patients: a qPCR and anaerobic culture assessment | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Reino Unido | pt_PT |
| oaire.citation.endPage | 9 | pt_PT |
| oaire.citation.startPage | 1 | pt_PT |
| oaire.citation.title | International Endodontic Journal | pt_PT |
| person.familyName | Manso | |
| person.familyName | Manuel Baptista Cabeda | |
| person.givenName | Maria da Conceição | |
| person.givenName | José | |
| person.identifier.ciencia-id | 5A18-502F-4844 | |
| person.identifier.ciencia-id | 3F17-D327-35C3 | |
| person.identifier.orcid | 0000-0002-1774-1337 | |
| person.identifier.orcid | 0000-0002-5117-2326 | |
| person.identifier.rid | D-7212-2013 | |
| person.identifier.scopus-author-id | 57196702148 | |
| rcaap.cv.cienciaid | B511-73B3-087A | Ana Moura Teles | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 7dff8d33-6bfb-496b-b734-a94146c562e6 | |
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| relation.isAuthorOfPublication.latestForDiscovery | c928b66f-c6b6-40b1-80ff-9831007c88eb |