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Degree of contamination of Gutta-Percha points by Staphylococcus aureus (MRSA/MSSA) strains

2024-08-06Proposta de investigação Acesso aberto
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datacite.subject.fosCiências Médicas
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorMoura Teles, Ana
dc.contributor.authorPina, Cristina
dc.contributor.authorLopes Cardoso, Inês
dc.contributor.authorTramontana, Antea
dc.contributor.authorCardoso, Miguel
dc.contributor.authorDuarte, Ana Sofia
dc.contributor.authorBartolomeu, Maria
dc.contributor.authorNoites, Rita
dc.date.accessioned2026-05-11T14:38:41Z
dc.date.available2026-05-11T14:38:41Z
dc.date.issued2024-08-06
dc.description.abstractMethicillin-resistant Staphylococcus aureus (MRSA) is considered one of the most harmful bacteria to human health. Dentistry, like all healthcare disciplines, places great emphasis on preventing scenarios that may result in cross-infection. Although various tested and already used materials are suitable for filling the root canal system, Gutta-Percha (GP) remains the preferred and widely accepted gold standard. Objective: We performed an in vitro analysis of the contamination of GP points, regarding the strains of Methicillin-resistant (MRSA) and Methicillin-sensitive (MSSA) Staphylococcus aureus, using classical microbiology methods and molecular biology techniques. Methods: Gutta-Percha points of two different brands from opened packages (already in use for 1 month) were collected for analysis. The assessment involved incubating the GP points in Brain Heart Infusion (BHI) medium to detect microbial growth. Growing microorganisms were plated on a selective and differential chromogenic medium for MRSA/MSSA strains, and the identification of isolates was confirmed by Polymerase Chain Reaction (PCR). In the case of microbial growth, the GP point was submitted to a disinfection protocol. Results: From the 315 collected GP points, only 6 (1.9%) resulted in being positive for microbial growth. After confirmation by PCR, only one sample of the six GP points was contaminated by MRSA, and the remaining five were MSSA-contaminated. The disinfection protocol was effective in all contaminated GP points. Conclusions: The Gutta-Percha points from opened pre-sterilized packages showed a very low degree of contamination by MRSA/MSSA. However, the detection of MSSA and MRSA strains raises concerns about potential contamination in dental clinic environments, and this risk cannot be considered negligible.eng
dc.identifier.citationTeles, A. M., Pina, C., Cardoso, I. L., Tramontana, A., Cardoso, M., Duarte, A. S., Bartolomeu, M., & Noites, R. (2024). Degree of contamination of gutta-percha points by Staphylococcus aureus (MRSA/MSSA) strains. International Journal of Molecular Sciences, 25(16), 8566. https://doi.org/10.3390/ijms25168566
dc.identifier.doi10.3390/ijms25168566
dc.identifier.issn14220067, 16616596
dc.identifier.urihttp://hdl.handle.net/10284/15421
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relation.hasversionhttps://www.mdpi.com/1422-0067/25/16/8566
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectStaphylococcus aureus
dc.subjectMRSA/MSSA
dc.subjectGutta-Percha
dc.subjectContamination
dc.subjectEndodontics
dc.titleDegree of contamination of Gutta-Percha points by Staphylococcus aureus (MRSA/MSSA) strainseng
dc.typeresearch proposal
dspace.entity.typePublication
oaire.citation.issue16
oaire.citation.startPage8566
oaire.citation.titleInternational Journal of Molecular Sciences
oaire.citation.volume25
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePina
person.familyNameLopes Cardoso
person.givenNameCristina
person.givenNameInês
person.identifier.ciencia-idF21F-16B4-3715
person.identifier.orcid0000-0001-8841-714X
person.identifier.orcid0000-0002-0693-9831
person.identifier.ridM-7156-2013
person.identifier.scopus-author-id6504669461
person.identifier.scopus-author-id54973754300
relation.isAuthorOfPublicationc068b617-53ff-4e88-8602-a7a77e8e7d28
relation.isAuthorOfPublicationf39fa18f-2d23-4690-bfa2-f8ba53e40775
relation.isAuthorOfPublication.latestForDiscoveryc068b617-53ff-4e88-8602-a7a77e8e7d28

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