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Regeneração periodontal com recurso a proteínas derivadas da matriz de esmalte: revisão bibliográfica
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Abstract(s)
As proteínas derivadas da matriz de esmalte são compostas principalmente por proteínas amelogenina, particularmente pelos fragmentos de proteína de 20, 13 e 6 kDa, e há evidências de que uma pequena porção de ameloblastina é transferida durante a extração do derivado da matriz do esmalte. Os efeitos dos componentes específicos do EMD, a saber, amelogenina e ameloblastina, no comportamento celular in vitro concluíram que a expressão genética, a produção de proteínas, a proliferação e a diferenciação de vários tipos de células podem ser afetados e frequentemente aumentados pelo EMD.
Em particular, as células do ligamento periodontal assim como outros géneros de células osteoblásticas parecem diferenciar-se em relação à atividade de cementoblastos / osteoblastos. Por outro lado, fibroblastos gengivais, células foliculares, cementoblastos e condrócitos foram levados a exibir proliferação aumentada e diferenciação inibida após exposição ao derivado da matriz do esmalte.
O derivado da matriz do esmalte apresenta-se como um sistema biológico único e intrigante, que deve despertar a curiosidade de mais pesquisadores que lidam com química de proteínas, complexos de biologia molecular, dispositivos de entrega e regeneração de tecidos ou substratos de engenharia tecidular.
A aplicação de proteínas derivadas da matriz do esmalte na forma de emdogain® estabeleceu um padrão moderno para a terapia de regeneração periodontal. A combinação do derivado da matriz do esmalte exibiu melhorias adicionais nos parâmetros clínicos e radiológicos.
O presente trabalho pretende avaliar o efeito das proteínas derivadas da matriz do esmalte no tratamento regenerativo de defeitos intra-ósseos.
Enamel matrix derivative confirms that it is primarily composed of amelogenin proteins, particularly the 20, 13, and 6 kDa protein fragments, and there is evidence that a small presence of ameloblastin is transferred during enamel matrix derivative extraction. The effects of specific components of EMD, namely amelogenin and ameloblastin, on cell behavior in vitro have concluded that gene expression, protein production, proliferation, and differentiation of various cell types can be affected and often enhanced by EMD. In particular, periodontal ligament and osteoblastic cell types appear to differentiate toward cementoblast/osteoblast activity. On the other hand, gingival fibroblasts, follicle cells, cementoblasts, and chondrocytes have been driven to exhibit enhanced proliferation and inhibited differentiation upon exposure to enamel matrix derivative. Enamel matrix derivative presents itself as a unique and intriguing biological system, which should arouse the curiosity of more researchers dealing with protein chemistry, molecular biology complexes, delivery devices, and tissue regeneration or tissue engineering substrates. Application of enamel matrix proteins in the form of emdogain® has set a modern standard for periodontal regeneration therapy. The combination of enamel matrix derivative exhibited additional improvements in both clinical and radiological parameters. The present paper portrays the combined effect of enamel matrix derivative proteins in the management of intrabony defects.
Enamel matrix derivative confirms that it is primarily composed of amelogenin proteins, particularly the 20, 13, and 6 kDa protein fragments, and there is evidence that a small presence of ameloblastin is transferred during enamel matrix derivative extraction. The effects of specific components of EMD, namely amelogenin and ameloblastin, on cell behavior in vitro have concluded that gene expression, protein production, proliferation, and differentiation of various cell types can be affected and often enhanced by EMD. In particular, periodontal ligament and osteoblastic cell types appear to differentiate toward cementoblast/osteoblast activity. On the other hand, gingival fibroblasts, follicle cells, cementoblasts, and chondrocytes have been driven to exhibit enhanced proliferation and inhibited differentiation upon exposure to enamel matrix derivative. Enamel matrix derivative presents itself as a unique and intriguing biological system, which should arouse the curiosity of more researchers dealing with protein chemistry, molecular biology complexes, delivery devices, and tissue regeneration or tissue engineering substrates. Application of enamel matrix proteins in the form of emdogain® has set a modern standard for periodontal regeneration therapy. The combination of enamel matrix derivative exhibited additional improvements in both clinical and radiological parameters. The present paper portrays the combined effect of enamel matrix derivative proteins in the management of intrabony defects.
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Keywords
Guided tissue regeneration Enamel matrix derivative Infra-bony defects Guided tissue regeneration Enamel matrix derivative Infra-bony defects