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Simultaneous determination of amphetamine derivatives in human urine after SPE extraction and HPLC-UV analysis

2004Journal article Restricted access
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dc.contributor.authorSoares, M. E.
dc.contributor.authorCarvalho, Márcia
dc.contributor.authorCarmo, H.
dc.contributor.authorRemião, F.
dc.contributor.authorCarvalho, F.
dc.contributor.authorBastos, M. L.
dc.date.accessioned2021-07-01T16:16:00Z
dc.date.available2021-07-01T16:16:00Z
dc.date.issued2004
dc.description.abstractAmphetamine derivatives are a class of compounds increasingly abused as recreational drugs in various regions of the world. Although d-amphetamine (AMPH) and 3,4-methylenedioxymethamphetamine (MDMA, ecstasy) are among the most commonly used, the abuse of other designer drugs such as 4-bromo-2,5-dimethoxyphenethylamine (2C-B) and 4-methylthioamphetamine (4-MTA) and their involvement in acute intoxications has been increasingly reported. There is evidence that abusers ingest these compounds either alone or in combination and the respective monitoring is important for both legal and health care purposes in hospital emergency. In the present study a simple and clean solid-phase extraction procedure from urine of AMPH and MDMA, and their major metabolites p-hydroxyamphetamine (OH-AMPH) and methylenedioxyamphetamine (MDA) and 2C-B and 4-MTA was developed. Analysis was performed by HPLC-UV and the precision of the technique was between 2.9 and 5.3% for all compounds. For the overall procedure, the precision values were between 3.3 and 5.9%. Recoveries obtained from spiked urines at three concentration levels were better than 84 +/- 4% for the six compounds. The limit of detection of the method for the compounds (between 5.3 and 84.0 ng) enables their identification in urine after ingestion of fatal and non-fatal doses. The main advantages of the present method lie in its simple, clean and reliable SPE extraction method of the six amphetamine derivatives from urine followed by their simultaneous detection and quantification by liquid chromatography with UV detection.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/bmc.315pt_PT
dc.identifier.eissn1099-0801
dc.identifier.issn0269-3879
dc.identifier.urihttp://hdl.handle.net/10284/10005
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley & Sonspt_PT
dc.subjectDrugs of abusept_PT
dc.subjectDesigner drugspt_PT
dc.subjectecstasypt_PT
dc.subject4-bromo-2,5-dimethoxyphenethylaminept_PT
dc.subject4-methylthioamphetaminept_PT
dc.titleSimultaneous determination of amphetamine derivatives in human urine after SPE extraction and HPLC-UV analysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage131pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage125pt_PT
oaire.citation.titleBiomedical Chromatographypt_PT
oaire.citation.volume18pt_PT
person.familyNameCarvalho
person.givenNameMarcia
person.identifier2017111
person.identifier.ciencia-id8B10-171E-E63E
person.identifier.orcid0000-0001-9884-4751
person.identifier.ridD-5999-2013
person.identifier.scopus-author-id7201413997
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3837b828-ba57-47f7-a811-cce65e4922c6
relation.isAuthorOfPublication.latestForDiscovery3837b828-ba57-47f7-a811-cce65e4922c6

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