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Biological activities of portuguese propolis: protection against free radical-induced erythrocyte damage and inhibition of human renal cancer cell growth in vitro

2011Journal article Restricted access
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dc.contributor.authorValente, Maria J.
dc.contributor.authorBaltazar, Ana F.
dc.contributor.authorHenrique, Rui
dc.contributor.authorEstevinho, Letícia
dc.contributor.authorCarvalho, Márcia
dc.date.accessioned2021-07-02T12:31:28Z
dc.date.available2021-07-02T12:31:28Z
dc.date.issued2011
dc.description.abstractThis study reports for the first time the biological properties of Portuguese propolis. The antioxidant potential of propolis samples from Bornes (Northeast) and Fundão (Centre) regions of Portugal was evaluated by their ability to inhibit the 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis and lipid peroxidation in human erythrocytes. Bornes and Fundão propolis strongly protected the erythrocyte membrane from hemolysis (IC(50) of 6.3±0.7 and 10.4±2.7 μg/ml, respectively), in a time- and concentration-dependent manner. This effect was found to be significantly higher than that presented by ascorbic acid (IC(50) of 31.0±5.6 μg/ml). In addition, human erythrocytes treated with propolis extracts showed concentration-dependent decrease in levels of malondialdehyde, a breakdown product of lipid peroxidation. Propolis extracts were also assayed for their anticancer properties on human renal cell carcinoma (RCC). Primary cultures of normal and cancerous renal cells derived from RCC patients, in addition to A-498 cell line, were treated with propolis extracts (0-100 μg/ml). Cytotoxic and antiproliferative effects were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Propolis extracts exhibited selective toxicity against malignant cells compared to normal cells. In vitro RCC growth was strongly inhibited by Bornes and Fundão propolis in a concentration-dependent manner. Our results indicate that Portuguese propolis constitutes an excellent source of effective natural antioxidant and chemopreventive agents.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.fct.2010.10.001pt_PT
dc.identifier.issn0278-6915
dc.identifier.urihttp://hdl.handle.net/10284/10028
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectPortuguese propolispt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectErythrocytept_PT
dc.subjectRenal cell carcinomapt_PT
dc.subjectAntiproliferative activitypt_PT
dc.subjectCancer chemopreventionpt_PT
dc.titleBiological activities of portuguese propolis: protection against free radical-induced erythrocyte damage and inhibition of human renal cancer cell growth in vitropt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage92pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage86pt_PT
oaire.citation.titleFood and Chemical Toxicologypt_PT
oaire.citation.volume49pt_PT
person.familyNameCarvalho
person.givenNameMarcia
person.identifier2017111
person.identifier.ciencia-id8B10-171E-E63E
person.identifier.orcid0000-0001-9884-4751
person.identifier.ridD-5999-2013
person.identifier.scopus-author-id7201413997
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3837b828-ba57-47f7-a811-cce65e4922c6
relation.isAuthorOfPublication.latestForDiscovery3837b828-ba57-47f7-a811-cce65e4922c6

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