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Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

2015Journal article Restricted access
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dc.contributor.authorBarros, J.
dc.contributor.authorGrenho, Liliana
dc.contributor.authorFernandes, M.H.
dc.contributor.authorManuel, C.M.
dc.contributor.authorMelo, L.F.
dc.contributor.authorNunes, O.C.
dc.contributor.authorMonteiro, F.J.
dc.contributor.authorFerraz, Maria Pia
dc.date.accessioned2019-09-23T07:42:55Z
dc.date.available2019-09-23T07:42:55Z
dc.date.issued2015
dc.description.abstractNanohydroxyapatite possesses exceptional biocompatibility and bioactivity regarding bone cells and tissues, justifying its use as a coating material or as a bone substitute. Unfortunately, this feature may also encourage bacterial adhesion and biofilm formation. Surface functionalization with antimicrobials is a promising strategy to reduce the likelihood of bacterial infestation and colonization on medical devices. Chlorhexidine digluconate is a common and effective antimicrobial agent used for a wide range of medical applications. The purpose of this work was the development of a nanoHA biomaterial loaded with CHX to prevent surface bacterial accumulation and, simultaneously, with good cytocompatibility, for application in the medical field. CHX (5-1500 mg/L) was loaded onto nanoHA discs and the materials were evaluated for CHX adsorption and release profile, physic-chemical features, antibacterial activity against Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis, and cytocompatibility toward L929 fibroblasts. Results showed that the adsorption of CHX on nanoHA surface occurred by electrostatic interactions between the cationic group of CHX and the phosphate group of nanoHA. The release of CHX from CHX-loaded nanoHA showed a fast initial rate followed by a slower kinetics release, due to constraints caused by dilution and diffusion-limiting processes. NanoHA.50 to nanoHA.1500 showed strong anti-sessile activity, inhibiting bacterial adhesion and the biofilm formation. CHX-nanoHA caused a dose- and time-dependent inhibitory effect on the proliferation of fibroblasts for nanoHA.100 to nanoHA.1500. Cellular behavior on nanoHA.5 and nanoHA.50 was similar to control. Therefore, CHX-loaded nanoHA surfaces appear as a promising alternative to prevention of devices-related infections.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.colsurfb.2015.04.034pt_PT
dc.identifier.issn0927-7765
dc.identifier.urihttp://hdl.handle.net/10284/8005
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0927776515002520?via%3Dihubpt_PT
dc.subjectActin Cytoskeletonpt_PT
dc.subjectAdsorptionpt_PT
dc.subjectAnimalspt_PT
dc.subjectAnti-Bacterial Agentspt_PT
dc.subjectBacterial Adhesionpt_PT
dc.subjectBiocompatible Materialspt_PT
dc.subjectCell Line, Tumorpt_PT
dc.subjectChlorhexidinept_PT
dc.subjectDurapatitept_PT
dc.subjectEscherichia colipt_PT
dc.subjectMicrobial Sensitivity Testspt_PT
dc.subjectMicroscopy, Confocalpt_PT
dc.subjectMicroscopy, Electron, Scanningpt_PT
dc.subjectNanoparticlespt_PT
dc.subjectSpectroscopy, Fourier Transform Infraredpt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectStaphylococcus epidermidispt_PT
dc.titleAnti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatitept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage314pt_PT
oaire.citation.startPage305pt_PT
oaire.citation.titleColloids and Surfaces B: Biointerfacespt_PT
oaire.citation.volume130pt_PT
person.familyNameGrenho
person.familyNameFerraz
person.givenNameLiliana
person.givenNameMaria Pia
person.identifierR-000-M1P
person.identifier.ciencia-idB41C-D747-BDBF
person.identifier.ciencia-id7F16-B2FB-9AD4
person.identifier.orcid0000-0001-9476-2839
person.identifier.orcid0000-0002-0274-106X
person.identifier.ridI-4540-2015
person.identifier.ridM-6316-2013
person.identifier.scopus-author-id55159751400
person.identifier.scopus-author-id7102012951
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb26a2a2c-0484-4214-97b3-b7ea69b58419
relation.isAuthorOfPublication7ba2ac59-40af-4ba1-b13e-4447f1686208
relation.isAuthorOfPublication.latestForDiscovery7ba2ac59-40af-4ba1-b13e-4447f1686208

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