Repository logo
 
Publication

Hepatoprotective activity of polyhydroxylated 2-styrylchromones against tert-butylhydroperoxide induced toxicity in freshly isolated rat hepatocytes

2003Journal article Restricted access
Simple item page
dc.contributor.authorFernandes, Eduarda
dc.contributor.authorCarvalho, Márcia
dc.contributor.authorCarvalho, Félix
dc.contributor.authorSilva, Artur M. S.
dc.contributor.authorSantos, Clementina M. M.
dc.contributor.authorPinto, Diana C. G. A.
dc.contributor.authorCavaleiro, José A. S.
dc.contributor.authorBastos, Maria de Lourdes
dc.date.accessioned2021-06-30T15:19:58Z
dc.date.available2021-06-30T15:19:58Z
dc.date.issued2003
dc.description.abstract2-Styrylchromones are a novel class of chromones, vinylogues of flavones (2-phenylchromones), which have recently been found in nature. The best described property of almost every group of flavones and other flavonoids, especially the hydroxylated derivatives, is their capacity to act as antioxidants. Indeed there is a widely accepted view that the positive health effects of flavones are due to their antioxidant activity. As oxidative stress is a main cause of liver toxicity induced by several hepatotoxicants, agents with the ability to protect the liver against reactive pro-oxidant species may be therapeutically useful. The present study evaluated the possible protective activity of six new synthetic polyhydroxylated 2-styrylchromone derivatives against the pro-oxidant hepatotoxicity exerted by tert-butylhydroperoxide ( t-BHP) in freshly isolated rat hepatocytes. The cells were preincubated with the 2-styrylchromones in the final concentrations of 3.125, 12.5, 25, 50, 100, and 200 microM for 5 min before treatment with 1.0 mM t-BHP for 30 min (throughout this incubation period the cells were exposed to both compounds). The well-known antioxidant 3-hydroxyflavone (quercetin) was used as positive control. At the end of the 30-min incubation period, aliquots of cells suspensions were taken for measurement of lactate dehydrogenase leakage, thiobarbituric acid reactive substances, reduced glutathione, and oxidized glutathione contents. The tested compounds exhibited in vitro protective activity against t-BHP induced hepatotoxicity (1.0 mM, 30 min). Three of the tested compounds, at the concentrations of 3.125, 12.5, 25, and 50 microM, prevented the t-BHP induced glutathione depletion, lipid peroxidation, and cell death in freshly isolated rat hepatocytes to a comparable potency with that of quercetin.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00204-003-0480-9pt_PT
dc.identifier.eissn1432-0738
dc.identifier.issn0340-5761
dc.identifier.urihttp://hdl.handle.net/10284/9991
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.subject2-Styrylchromonespt_PT
dc.subjectTert- Butylhydroperoxidept_PT
dc.subjectHepatoprotective activitypt_PT
dc.subjectFreshly isolated rat hepatocytespt_PT
dc.titleHepatoprotective activity of polyhydroxylated 2-styrylchromones against tert-butylhydroperoxide induced toxicity in freshly isolated rat hepatocytespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage505pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage500pt_PT
oaire.citation.titleArchives of Toxicologypt_PT
oaire.citation.volume77pt_PT
person.familyNameCarvalho
person.givenNameMarcia
person.identifier2017111
person.identifier.ciencia-id8B10-171E-E63E
person.identifier.orcid0000-0001-9884-4751
person.identifier.ridD-5999-2013
person.identifier.scopus-author-id7201413997
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3837b828-ba57-47f7-a811-cce65e4922c6
relation.isAuthorOfPublication.latestForDiscovery3837b828-ba57-47f7-a811-cce65e4922c6

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Hepatoprotective activity of polyhydroxylated 2-styrylchromones.pdf
Size:
357.97 KB
Format:
Adobe Portable Document Format
Download

Collections

FCS - Artigos em Revistas Científicas Internacionais com Arbitragem Científica