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Oxidative stress, antioxidants and biomarkers: appreciation for analytical methods for health promotion

2023-06Journal article Open access
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dc.contributor.authorFerreira da Vinha, Ana
dc.contributor.authorSilva, Carla Sousa e
dc.contributor.authorCosta, Céu
dc.date.accessioned2023-06-29T09:59:12Z
dc.date.available2023-06-29T09:59:12Z
dc.date.issued2023-06
dc.description.abstractBecause of its extensive potential impact on human health, the determination of oxidative stress has been the main goal of study in several studies over the last few decades. The current work is a bibliographic review of the main methods of assessing oxidative stress in humans, taking into account the adverse effects it can do to individual quality of life and public health. To situate the theme, initially was described the concept of oxidative stress and the main diseases associated with it. Afterwards was presented the principal species of free radicals responsible for the oxidation, the antioxidant agents, enzymatic and non enzymatic , and the main bio marker of evaluation of oxidative stress. Regarding the analytical methods, it was proceeded by it's grouping according with the analyte in cause. In this regard of the reactive species of oxygen (ROS) and nitrogen (RNS), are presented the principal fluorescent and chemiluminescent probes, more concretely lucigenin, dihydroethidium DHE, and MitoSox, all characterized of the measurement of the superoxide, just like the relative to the determination of hydrogen peroxide as 2', 7'- dichlorodihydrofluorescein diacetate (DCFH-DA), boronate and Amplex Red. About the RNS, are shown the 4,5-diaminofluorescein (DAF2-DA) and the dihydroethidium fluorescent probes as a method of determination of the level of NO and peroxynitrite, respectively. For biomarkers, the current work, presents the analysis methods dedicated to the detection of F2-isoprostanes, lipid hydroperoxides and malondialdehyde (MDA) associated with lipid peroxidation. Chromatographic methods, hyphenated with chemiluminescence spectroscopy, fluorescence, and mass spectrometry (MS/MS), and electrochemical detection (ECD) is the most frequently used along with the enzymatic immunoassays (ELISA). With regard to antioxidants, diversity methodologies is most evident, although the methods overridden by liquid chromatography.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVinha, A.F., Sousa, C., Costa, C. (2023). Oxidative stress, antioxidants and biomarkers: appreciation for analytical methods for health promotion. nternational Academic Research Journal of Internal Medicine & Public Health, 4(3), 47-55. DOI: 10.47310/iarjimph.2023.v04i03.009pt_PT
dc.identifier.doi10.47310/iarjimph.2023.v04i03.009pt_PT
dc.identifier.eissn2709-331X
dc.identifier.issn2709-3301
dc.identifier.urihttp://hdl.handle.net/10284/11951
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherIARCON International LLPpt_PT
dc.relation.publisherversionhttps://www.iarconsortium.org/article/oxidative-stress-antioxidants-and-biomarkers-appreciation-for-analytical-methods-for-health-promotion-2440/pt_PT
dc.subjectOxidative stresspt_PT
dc.subjectReactive oxygen and nitrogen speciespt_PT
dc.subjectBiomarkerspt_PT
dc.subjectAntioxidantspt_PT
dc.subjectAnalytical procedurespt_PT
dc.titleOxidative stress, antioxidants and biomarkers: appreciation for analytical methods for health promotionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage55pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage47pt_PT
oaire.citation.titleInternational Academic Research Journal of Internal Medicine & Public Healthpt_PT
oaire.citation.volume4pt_PT
person.familyNameMendes Ferreira da Vinha
person.familyNameSilva
person.familyNameCosta
person.givenNameAna Cristina
person.givenNameCarla
person.givenNameCéu
person.identifier.ciencia-id011A-FCBD-DC66
person.identifier.ciencia-id2E1D-05DF-E0DB
person.identifier.orcid0000-0002-6116-9593
person.identifier.orcid0000-0001-6467-4766
person.identifier.orcid0000-0003-3766-2887
person.identifier.ridD-8159-2013
person.identifier.scopus-author-id55599276500
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb09696c0-d5ea-4218-a5e4-63a3f6288d83
relation.isAuthorOfPublication741eff47-ffdf-4a5f-b763-3ada0062b1b8
relation.isAuthorOfPublication8ee2ddb5-6a33-43b7-9950-1cde2daa5e7a
relation.isAuthorOfPublication.latestForDiscovery741eff47-ffdf-4a5f-b763-3ada0062b1b8

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