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Bacterial contamination of Gutta-Percha points from different brands and the efficacy of a chairside disinfection protocol

2020-11-09Documento de Trabalho Acesso aberto
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datacite.subject.fosCiências Médicas
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorBracciale, Francesca
dc.contributor.authorMarino, Nicole
dc.contributor.authorNoronha, Anariely
dc.contributor.authorManso, Maria da Conceição
dc.contributor.authorGavinha, Sandra
dc.contributor.authorLopes Cardoso, Inês
dc.contributor.authorPina, Cristina
dc.contributor.authorMoura Teles, Ana
dc.date.accessioned2026-05-11T11:22:14Z
dc.date.available2026-05-11T11:22:14Z
dc.date.issued2020-11-09
dc.description.abstractObjective: To evaluate the bacterial contamination of different brands of Gutta-Percha (GP) points routinely used in clinical practice and the efficacy of a chairside disinfection protocol with sodium hypochlorite. Methods: GP points (n=240), in sizes A, B, C, D, K15, K20, K25, K30, K35, K40, F1, F2, F3 (Dentsply®, Proclinic®, ProTaper® and R&S®), were randomly sampled from commercial packages already in use. These were added directly to Fluid Thioglycolate Medium (one GP point per tube) and incubated at 37ºC for 21 days. During this period, the presence/absence of turbidity was evaluated. To evaluate the efficacy of a chairside disinfection protocol, all detected contaminated GP points were immersed for 1 minute in 10 mL of 5.25% sodium hypochlorite, followed by 5 minutes in 10 mL of detergent solution (3% Tween 80 and 5% sodium thiosulfate) and a final rinse with 10 mL of sterile distilled water and incubated. The data was analysed using the chi-square test and differences between characteristics of dichotomic variables were performed using the binomial test. The significance level was set at P<0.05. Results: Bacterial growth was observed in 22.9% of the total study samples. Dentsply® and R&S® showed the highest level of contamination, 47.3% each, although without significant differences to the other commercial brands. The most contaminated GP point size was K30 (16.4%). The chairside disinfection protocol was effective in disinfection of 76.4% of GP points (P<0.001). Conclusion: A real small number of GP points in clinical use harboured bacteria, including after the Chairside Disinfection Protocol that, anyway, proved to be effective. No significant difference was observed between tested commercial brands.eng
dc.identifier.citationBracciale, F., Marino, N., Noronha, A., Manso, M. C., Gavinha, S., Cardoso, I. L., Pina, C., & Teles, A. M. (2020). Bacterial contamination of gutta-percha points from different brands and the efficacy of a chairside disinfection protocol. European Endodontic Journal, 5(3), 282–287. https://doi.org/10.14744/eej.2020.44265
dc.identifier.doi10.14744/eej.2020.44265
dc.identifier.issn25480839
dc.identifier.urihttp://hdl.handle.net/10284/15409
dc.language.isoeng
dc.peerreviewedyes
dc.relation.hasversionhttps://www.eurendodj.com/index.php/pub/article/view/122
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectContamination
dc.subjectDisinfection protocol
dc.subjectEndodontic treatment
dc.subjectGutta-Percha points
dc.subjectRoot canal filling
dc.subjectSecondary endodontic infection
dc.titleBacterial contamination of Gutta-Percha points from different brands and the efficacy of a chairside disinfection protocoleng
dc.typeworking paper
dspace.entity.typePublication
oaire.citation.endPage287
oaire.citation.startPage282
oaire.citation.titleEuropean Endodontic Journal
oaire.citation.volume3
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameManso
person.familyNameGavinha
person.familyNameLopes Cardoso
person.familyNamePina
person.givenNameMaria da Conceição
person.givenNameSandra
person.givenNameInês
person.givenNameCristina
person.identifier.ciencia-id5A18-502F-4844
person.identifier.ciencia-id0A14-1FF4-3383
person.identifier.ciencia-idF21F-16B4-3715
person.identifier.orcid0000-0002-1774-1337
person.identifier.orcid0000-0002-2430-1241
person.identifier.orcid0000-0002-0693-9831
person.identifier.orcid0000-0001-8841-714X
person.identifier.ridD-7212-2013
person.identifier.ridM-7156-2013
person.identifier.scopus-author-id57196702148
person.identifier.scopus-author-id35758590200
person.identifier.scopus-author-id54973754300
person.identifier.scopus-author-id6504669461
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relation.isAuthorOfPublication.latestForDiscoveryf39fa18f-2d23-4690-bfa2-f8ba53e40775

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