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Modified rose bengal assay for surface hydrophobicity evaluation of cationic solid lipid nanoparticles (cSLN)

2012-04Journal article Restricted access
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dc.contributor.authorDoktorovova, S.
dc.contributor.authorShegokar, R.
dc.contributor.authorMartins-Lopes, P.
dc.contributor.authorSilva, A.M.
dc.contributor.authorLopes, Carla Martins
dc.contributor.authorMüller, R.H.
dc.contributor.authorSouto, Eliana B.
dc.date.accessioned2020-10-14T09:29:44Z
dc.date.available2020-10-14T09:29:44Z
dc.date.issued2012-04
dc.date.updated2020-07-24T16:15:24Z
dc.description.abstractSurface hydrophobicity of nanocarriers influences protein binding and subsequently fate of nanoparticles in blood circulation. Therefore, characterization of surface hydrophobicity of nanocarriers provides important preclinical information. Here, a modified classical adsorption method for the needs of characterization of cationic solid lipid nanoparticles (cSLN) was developed. We have identified possible method limitations that should be considered when performing the analysis, i.e. the problems associated with particle separation from the dispersion and their own absorbance in visible spectrum. We propose two modified methods for performing the assay overcoming the stated limitations. We also discuss here evaluation by different approaches (calculation of binding constants or partitioning quotient) and their suitability for the prepared cSLN formulation. Overall, we confirmed that our modified adsorption method can provide useful information about surface properties of (cationic) SLN, however, performing and evaluation of the assay need special attention in order to obtain the desired results.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ejps.2011.12.016pt_PT
dc.identifier.eid2-s2.0-84857910609
dc.identifier.issn0928-0987
dc.identifier.slugcv-prod-336975
dc.identifier.urihttp://hdl.handle.net/10284/9056
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.subjectSolid lipid nanoparticlespt_PT
dc.subjectRose Bengalpt_PT
dc.subjectAdsorptionpt_PT
dc.subjectSurface hydrophobicitypt_PT
dc.titleModified rose bengal assay for surface hydrophobicity evaluation of cationic solid lipid nanoparticles (cSLN)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage612pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage606pt_PT
oaire.citation.titleEuropean Journal of Pharmaceutical Sciencespt_PT
oaire.citation.volume45pt_PT
person.familyNameLopes
person.familyNameSouto
person.givenNameCarla
person.givenNameEliana B.
person.identifier.ciencia-id901D-160C-633E
person.identifier.ciencia-id8D1B-21CC-90F7
person.identifier.orcid0000-0001-5080-032X
person.identifier.orcid0000-0002-9737-6017
person.identifier.ridM-4689-2016
person.identifier.ridL-9813-2014
person.identifier.scopus-author-id26649517700
person.identifier.scopus-author-id8839435500
rcaap.cv.cienciaid901D-160C-633E | Carla Martins Lopes
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd2a48cfe-9258-4916-99be-bbcb710f6605
relation.isAuthorOfPublicationb9f031fa-7f68-4196-b9c6-9f17cd542f12
relation.isAuthorOfPublication.latestForDiscoveryb9f031fa-7f68-4196-b9c6-9f17cd542f12

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